Assessing Antigen Presentation on the Surface of Plasmodium falciparum-Infected Erythrocytes by Photoactivated Localization Microscopy (PALM)

Christos Karathanasis; Cecilia P Sanchez; Mike Heilemann; Michael Lanzer

doi:10.1007/978-1-0716-2189-9_34

Assessing Antigen Presentation on the Surface of Plasmodium falciparum-Infected Erythrocytes by Photoactivated Localization Microscopy (PALM)

Methods Mol Biol. 2022:2470:457-466. doi: 10.1007/978-1-0716-2189-9_34.

Authors

Christos Karathanasis¹, Cecilia P Sanchez², Mike Heilemann^{1

3}, Michael Lanzer⁴

Affiliations

¹ Institute for Physical and Theoretical Chemistry, Goethe-University Frankfurt, Frankfurt, Germany.
² Center of Infectious Diseases, Parasitology, Universitätsklinikum Heidelberg, Heidelberg, Germany.
³ BioQuant-Center for Quantitative Biology, Heidelberg University, Heidelberg, Germany.
⁴ Center of Infectious Diseases, Parasitology, Universitätsklinikum Heidelberg, Heidelberg, Germany. michael.lanzer@med.uni-heidelberg.de.

PMID: 35881366
DOI: 10.1007/978-1-0716-2189-9_34

Abstract

Super-resolution microscopy in the form of photoactivated localization microscopy (PALM) offers the possibility of counting single molecules in a cell, a cellular compartment or a molecular complex. PALM can, therefore, underpin molecular and biochemical processes with a numeric and stoichiometric understanding of the interacting players. Here, we introduce the physical principles underlying PALM and provide a step-by-step protocol of how to apply PALM to questions related to the biology and pathophysiology of P. falciparum and other malaria parasites.

Keywords: PALM; PfEMP1; Photoactivated localization microscopy; STORM; Single-molecule counting.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigen Presentation
Erythrocytes / parasitology
Humans
Malaria, Falciparum* / parasitology
Microscopy
Plasmodium falciparum*
Protozoan Proteins / chemistry

Substances

Protozoan Proteins