Delineating COVID-19 immunological features using single-cell RNA sequencing

Wendao Liu; Johnathan Jia; Yulin Dai; Wenhao Chen; Guangsheng Pei; Qiheng Yan; Zhongming Zhao

doi:10.1016/j.xinn.2022.100289

Delineating COVID-19 immunological features using single-cell RNA sequencing

Innovation (Camb). 2022 Sep 13;3(5):100289. doi: 10.1016/j.xinn.2022.100289. Epub 2022 Jul 21.

Authors

Wendao Liu^{1

2}, Johnathan Jia^{1

2}, Yulin Dai², Wenhao Chen^{3

4}, Guangsheng Pei², Qiheng Yan², Zhongming Zhao^{1

2

5

6}

Affiliations

¹ The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA.
² Center for Precision Health, School of Biomedical Informatics, the University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
³ Immunobiology and Transplant Science Center, Department of Surgery, Houston Methodist Research Institute and Institute for Academic Medicine, Houston Methodist Hospital, Houston, TX 77030, USA.
⁴ Department of Surgery, Weill Cornell Medicine, Cornell University, New York, NY 10065, USA.
⁵ Human Genetics Center, School of Public Health, the University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
⁶ Department of Biomedical Informatics, Vanderbilt University Medical Center, Nashville, TN 37203, USA.

Abstract

Understanding the molecular mechanisms of coronavirus disease 2019 (COVID-19) pathogenesis and immune response is vital for developing therapies. Single-cell RNA sequencing has been applied to delineate the cellular heterogeneity of the host response toward COVID-19 in multiple tissues and organs. Here, we review the applications and findings from over 80 original COVID-19 single-cell RNA sequencing studies as well as many secondary analysis studies. We describe that single-cell RNA sequencing reveals multiple features of COVID-19 patients with different severity, including cell populations with proportional alteration, COVID-19-induced genes and pathways, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection in single cells, and adaptation of immune repertoire. We also collect published single-cell RNA sequencing datasets from original studies. Finally, we discuss the limitations in current studies and perspectives for future advance.

Publication types

Review

Abstract

Publication types

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