Protein-protein interaction techniques to investigate post-translational regulation of carotenogenesis

Abstract

Carotenoids are indispensable to plants. The regulatory mechanisms underlying carotenoid metabolism have been subjected to intensive investigation. Post-translational regulation is critically important to rapidly modulate enzyme protein level and activity in fine-tuning carotenoid production in living organisms. However, the regulatory controls at the post-translational level are poorly understood. This chapter highlights the recent advances in this area of research and presents the protein-protein interaction protocols to study the post-translational regulation of carotenogenesis.

Keywords: BiFC; Carotenogenesis; Post-translational regulation; Protein–protein interaction; Pull-down; Y2H.