Complex network analysis of slaughterhouse waste anaerobic digestion: From failure to success of long-term operation

Tergel Dalantai; Chaeyoung Rhee; Dae Wook Kim; Sung Il Yu; Juhee Shin; Jin Mi Triolo; Seung Gu Shin

doi:10.1016/j.biortech.2022.127673

Complex network analysis of slaughterhouse waste anaerobic digestion: From failure to success of long-term operation

Bioresour Technol. 2022 Oct:361:127673. doi: 10.1016/j.biortech.2022.127673. Epub 2022 Jul 22.

Authors

Tergel Dalantai¹, Chaeyoung Rhee¹, Dae Wook Kim¹, Sung Il Yu², Juhee Shin¹, Jin Mi Triolo³, Seung Gu Shin⁴

Affiliations

¹ Department of Energy Engineering, Future Convergence Technology Research Institute, Gyeongsang National University, 33 Dongjin-ro, Jinju, Gyeongnam 52828, Republic of Korea.
² Department of Energy Engineering, Future Convergence Technology Research Institute, Gyeongsang National University, 33 Dongjin-ro, Jinju, Gyeongnam 52828, Republic of Korea; School of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology, 50 UNIST-gil, Ulju-gun, Ulsan 44919, Republic of Korea.
³ Department of Energy Engineering, Future Convergence Technology Research Institute, Gyeongsang National University, 33 Dongjin-ro, Jinju, Gyeongnam 52828, Republic of Korea. Electronic address: jinmitriolo@gnu.ac.kr.
⁴ Department of Energy Engineering, Future Convergence Technology Research Institute, Gyeongsang National University, 33 Dongjin-ro, Jinju, Gyeongnam 52828, Republic of Korea; Department of Energy System Engineering, Gyeongsang National University, 33 Dongjin-ro, Jinju, Gyeongnam 52828, Republic of Korea.

PMID: 35878765
DOI: 10.1016/j.biortech.2022.127673

Abstract

The study explored slaughterhouse waste (SHW) as prime feedstock associated with and without supplement of an external slowly degradable lignocellulosic carbon source to overcome the synergistic co-inhibitions of ammonia and fatty acids. Long-term solid-state digestion (SSD) and liquid-state digestion (LSD) were investigated using a mixture of pork liver and fat. At 2.0 g volatile solids (VS) L^-1 d^-1 of organic loading rate (OLR), the two reactors of SSD experienced operational instability due to ammonia inhibition and volatile fatty acid (VFA) accumulation while LSD successfully produced 0.725 CH₄ L CH₄ g^-1VS during 197 d of working days under unfavorable condition with high total ammonia nitrogen (>4.7 g/L) and VFAs concentration (>1.9 g/L). The network analysis between complex microflora and operational parameters provided an insight for sustainable biogas production using SHW. Among all, hydrogenotrophic methanogens have shown better resistance than acetoclastic methanogens.

Keywords: Ammonia; Co-inhibition; Network analysis; Organic loading rate; Solid-state digestion.

MeSH terms

Abattoirs*
Ammonia
Anaerobiosis
Biofuels
Bioreactors*
Methane

Substances

Ammonia
Biofuels
Methane