Effect of Fumonisin B1 on Proliferation and Apoptosis of Intestinal Porcine Epithelial Cells

Toxins (Basel). 2022 Jul 9;14(7):471. doi: 10.3390/toxins14070471.

Abstract

Fumonisin B1 (FB1), which is a mycotoxin produced by Fusarium moniliforme and Fusarium rotarum, has a number of toxic effects in animals. Moldy feed containing FB1 can damage the intestine. In this study, we used intestinal porcine epithelial cells (IPEC-J2) as an in vitro model to explore the effects of FB1 on cell cycle and apoptosis. The results showed that IPEC-J2 cells treated with 10, 20, and 40 μg/mL FB1 for 48 h experienced different degrees of damage manifested as decreases in cell number and viability, as well as cell shrinkage and floating. In addition, FB1 reduced cell proliferation and the mRNA and protein expression of proliferating cell nuclear antigen (PCNA), cyclin-dependent kinase 2 (CDK2), CDK4, cyclinD1, and cyclinE1. FB1 blocked the cell cycle in the G1 phase. FB1 also induced mitochondrial pathway apoptosis, reduced mitochondrial membrane potential, and promoted mRNA and protein expression of Caspase3, Caspase9, and Bax. The findings suggest that FB1 can induce IPEC-J2 cell damage, block the cell cycle, and promote cell apoptosis.

Keywords: Fumonisin B1; apoptosis; cell proliferation; intestinal porcine epithelial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Cell Count
  • Cell Line
  • Cell Proliferation
  • Epithelial Cells
  • Fumonisins* / metabolism
  • Fumonisins* / toxicity
  • Intestines
  • RNA, Messenger / metabolism
  • Swine

Substances

  • Fumonisins
  • RNA, Messenger
  • fumonisin B1

Grants and funding

This work was supported by the Scientific Research Project of Hunan Provincial Education Department (19A229), The Natural Science Foundation of Hunan Province, China (2022JJ30022, 2022JJ30293), Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines (2017TP1014).