Establishment and characterization of IPS-OGC-C1: a novel induced pluripotent stem cell line from healthy human ovarian granulosa cells

Zhiqiang Wang; Xiaojia Hu; Qiufen He; Jingbo Lai; Ruolang Pan; Jing Zheng; Ye Chen

doi:10.1007/s13577-022-00757-7

Establishment and characterization of IPS-OGC-C1: a novel induced pluripotent stem cell line from healthy human ovarian granulosa cells

Hum Cell. 2022 Sep;35(5):1612-1620. doi: 10.1007/s13577-022-00757-7. Epub 2022 Jul 25.

Authors

Zhiqiang Wang^#^{1

2}, Xiaojia Hu^#^{1

2}, Qiufen He^#^{2

3}, Jingbo Lai^{1

2}, Ruolang Pan⁴, Jing Zheng^{5

6}, Ye Chen^{7

8}

Affiliations

¹ Department of Genetics, Department of Genetic and Metabolic Disease, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310052, Zhejiang, China.
² Zhejiang Provincial Key Laboratory of Genetic and Developmental Disorders, Institute of Genetics, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
³ Reproductive Medical Center, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310004, Zhejiang, China.
⁴ Zhejiang Provincial Key Laboratory of Cell-Based Drug and Applied Technology Development, Hangzhou, 310023, Zhejiang, China.
⁵ Department of Genetics, Department of Genetic and Metabolic Disease, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310052, Zhejiang, China. candy88zj@zju.edu.cn.
⁶ Zhejiang Provincial Key Laboratory of Genetic and Developmental Disorders, Institute of Genetics, Zhejiang University, Hangzhou, 310058, Zhejiang, China. candy88zj@zju.edu.cn.
⁷ Department of Genetics, Department of Genetic and Metabolic Disease, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310052, Zhejiang, China. yechency@zju.edu.cn.
⁸ Zhejiang Provincial Key Laboratory of Genetic and Developmental Disorders, Institute of Genetics, Zhejiang University, Hangzhou, 310058, Zhejiang, China. yechency@zju.edu.cn.

^# Contributed equally.

PMID: 35876985
DOI: 10.1007/s13577-022-00757-7

Abstract

Ovarian granulosa cell (OGC) is a critical somatic component of the ovary, which provides physical support and the microenvironment required for the developing oocyte. Human OGCs are easy to obtain and culture as a by-product of follicular aspiration performed during in vitro fertilization (IVF) procedures. Therefore, OGCs offer a potent cell source to generate induced pluripotent stem cells (iPSCs). This study established a novel OGCs-derived iPSC cell line from the follicular fluid of a healthy female donor with a Chinese Han genetic background and named it IPS-OGC-C1. IPS-OGC-C1 was verified for embryonic stem cell morphology, cell marker expression, alkaline phosphatase (AP) activity, transcriptomic profile, and pluripotency capability in developing all three embryonic germ layers in vivo and in vitro.

Keywords: Induced pluripotent stem cells; human ovarian granulosa cells; reprogramming.

MeSH terms

Cell Differentiation / genetics
Embryonic Stem Cells
Female
Granulosa Cells
Humans
Induced Pluripotent Stem Cells*
Oocytes

Abstract

MeSH terms

Grants and funding