Effect of daflon-500®, a flavonoid compound on chlorpyriphos-induced oxidative changes in the hypophysis and testes in adult male rats

Aishat O Olatunji; Joseph O Ayo; Mohammed M Suleiman; Suleiman F Ambali; Muftau Shittu; Ganiu J Akorede; Lukman O Raji; Jamila A Atata; Khalid T Biobaku; Mistura O Azeez

doi:10.1007/s43188-021-00120-2

Effect of daflon-500^®, a flavonoid compound on chlorpyriphos-induced oxidative changes in the hypophysis and testes in adult male rats

Toxicol Res. 2022 Jan 10;38(3):345-353. doi: 10.1007/s43188-021-00120-2. eCollection 2022 Jul.

Affiliations

¹ Veterinary Pharmacology and Toxicology, University of Ilorin, Ilorin, Kwara Nigeria.
² Veterinary Physiology, Ahmadu Bello University, Zaria, Kaduna Nigeria.
³ Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Kaduna Nigeria.
⁴ Theriogenology and Production, University of Ilorin, Ilorin, Kwara Nigeria.
⁵ Veterinary Pathology, University of Ilorin, Ilorin, Kwara Nigeria.
⁶ Veterinary Physiology and Biochemistry, University of Ilorin, Ilorin, Kwara Nigeria.

Abstract

Alteration of redox status is one of the molecular pathways commonly associated with pesticide toxicity. Antioxidants, including those obtained from plant phenolics, have been shown to mitigate pesticide-induced cellular injury. The present study was aimed at evaluating the effect of daflon-500 ^® , a flavonoid compound on sub-chronic chlorpyriphos-evoked changes in antioxidant and biochemical parameters in the hypophysis and testes of adult male rats. Twenty-five male albino rats were randomly divided into 5 groups of 5 animals each. Group I (DW) received distilled water (2 ml/kg); group II (SO) was dosed with soya oil (2 ml/kg); Group III (DAF) received daflon-500 ^® at 1000 mg/kg ~ 1/5th of LD50 (≥ 5000 mg/kg); group IV (CP) was administered chlorpyriphos at 7.74 mg/kg ~ 1/10th of LD₅₀ (77.4 mg/kg) while group V (DAF + CP) was previously treated with daflon-500 ^® (1000 mg/kg) and then exposed to CP (7.74 mg/kg), 30 min later. Daily oral regimen administration was done for 60 days after which the animals were sacrificed by cervical venesection after light chloroform anesthesia. The hypophysis and testicular tissues were harvested, and their homogenates were analyzed for malondialdehyde, catalase and superoxide dismutase, and acetylcholinesterase levels. A significant increase in the hypophysis and testicular MDA concentrations, coupled with a decrease in the SOD, CAT, and AChE activities were observed in the CP group. The levels of these oxidative and biochemical parameters were alleviated in the group pretreated with Daflon-500 ^® . Results of this study demonstrated that pre-treatment with Daflon-500 ^® mitigated CP-induced alterations in oxidative and biochemical parameters apparently due to the antioxidant effect of the flavonoid compound.

Keywords: Antioxidants; Chlorpyriphos; Daflon-500; Hypophysis; Oxidative stress; Testes.