Aptamer-based biosensors for virus protein detection

Beibei Lou; Yanfei Liu; Meilin Shi; Jun Chen; Ke Li; Yifu Tan; Liwei Chen; Yuwei Wu; Ting Wang; Xiaoqin Liu; Ting Jiang; Dongming Peng; Zhenbao Liu

doi:10.1016/j.trac.2022.116738

Aptamer-based biosensors for virus protein detection

Trends Analyt Chem. 2022 Dec:157:116738. doi: 10.1016/j.trac.2022.116738. Epub 2022 Jul 19.

Authors

Beibei Lou¹, Yanfei Liu², Meilin Shi³, Jun Chen¹, Ke Li¹, Yifu Tan², Liwei Chen², Yuwei Wu¹, Ting Wang¹, Xiaoqin Liu², Ting Jiang², Dongming Peng⁴, Zhenbao Liu^{1

5}

Affiliations

¹ Department of Pharmaceutics, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410013, Hunan Province, PR China.
² Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, Hunan Province, PR China.
³ School of Medical Imaging, Xuzhou Medical University, Xuzhou, Jiangsu, 221004, PR China.
⁴ Department of Medicinal Chemistry, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, PR China.
⁵ Molecular Imaging Research Center of Central South University, Changsha, 410008, Hunan, PR China.

Abstract

Virus threatens life health seriously. The accurate early diagnosis of the virus is vital for clinical control and treatment of virus infection. Aptamers are small single-stranded oligonucleotides (DNAs or RNAs). In this review, we summarized aptasensors for virus detection in recent years according to the classification of the viral target protein, and illustrated common detection mechanisms in the aptasensors (colorimetry, fluorescence assay, surface plasmon resonance (SPR), surface-enhanced raman spectroscopy (SERS), electrochemical detection, and field-effect transistor (FET)). Furthermore, aptamers against different target proteins of viruses were summarized. The relationships between the different biomarkers of the viruses and the detection methods, and their performances were revealed. In addition, the challenges and future directions of aptasensors were discussed. This review will provide valuable references for constructing on-site aptasensors for detecting viruses, especially the SARS-CoV-2.

Keywords: Aptamer; Aptasensor; Detection; Virus.

Publication types

Review