Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC

Aftab Alam; Sarah Comer; Eric Levanduski; Prasenjit Dey

doi:10.1016/j.xpro.2022.101563

Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC

STAR Protoc. 2022 Jul 18;3(3):101563. doi: 10.1016/j.xpro.2022.101563. eCollection 2022 Sep 16.

Authors

Aftab Alam¹, Sarah Comer¹, Eric Levanduski¹, Prasenjit Dey²

Affiliations

¹ Department of Immunology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA.
² Department of Immunology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA. Electronic address: prasenjit.dey@roswellpark.org.

Abstract

Innate lymphoid cells 2 (ILC2) play a significant role in the tumorigenesis of pancreatic ductal adenocarcinoma (PDAC). An important aspect of ILC2-mediated tumorigenesis is the expansion of the resident ILC2 and simultaneous recruitment of the peripheral ILC2. Here, we describe a protocol for isolation, enrichment, and DiD labeling of ILC2 for in vivo tracking of ILC2s in the mouse. Further, we describe steps for the adoptive transfer of ILC2 to a recipient mouse model of PDAC. For complete details on the use and execution of this protocol, please refer to Alam et al. (2022).

Keywords: Cancer; Cell Biology; Cell culture; Cell isolation; Immunology; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Adoptive Transfer
Animals
Carcinogenesis
Carcinoma, Pancreatic Ductal* / therapy
Disease Models, Animal
Immunity, Innate
Lymphocytes
Mice
Pancreatic Neoplasms*

Abstract

Publication types

MeSH terms

Grants and funding