Superior Haplotypes for Early Root Vigor Traits in Rice Under Dry Direct Seeded Low Nitrogen Condition Through Genome Wide Association Mapping

Annamalai Anandan; Siddharth Panda; S Sabarinathan; Anthony J Travis; Gareth J Norton; Adam H Price

doi:10.3389/fpls.2022.911775

Superior Haplotypes for Early Root Vigor Traits in Rice Under Dry Direct Seeded Low Nitrogen Condition Through Genome Wide Association Mapping

Front Plant Sci. 2022 Jul 8:13:911775. doi: 10.3389/fpls.2022.911775. eCollection 2022.

Authors

Annamalai Anandan^{1

2}, Siddharth Panda^{1

3}, S Sabarinathan¹, Anthony J Travis⁴, Gareth J Norton⁴, Adam H Price⁴

Affiliations

¹ Crop Improvement Division, Indian Council of Agricultural Research (ICAR)-National Rice Research Institute (NRRI), Cuttack, India.
² Indian Council of Agricultural Research (ICAR)-Indian Institute of Seed Science (IISS), Bengaluru, India.
³ Department of Plant Breeding and Genetics, Odisha University of Agriculture & Technology, Bhubaneswar, India.
⁴ School of Biological Sciences, University of Aberdeen, Aberdeen, United Kingdom.

Abstract

Water and land resources have been aggressively exploited in the recent decades to meet the growing demands for food. The changing climate has prompted rice scientists and farmers of the tropics and subtropics to adopt the direct seeded rice (DSR) system. DSR system of rice cultivation significantly reduces freshwater consumption and labor requirements, while increasing system productivity, resource use efficiency, and reducing greenhouse gas emissions. Early root vigor is an essential trait required in an ideal DSR system of rice cultivation to ensure a good crop stand, adequate uptake of water, nutrients and compete with weeds. The aus subpopulation which is adapted for DSR was evaluated to understand the biology of early root growth under limited nitrogen conditions over two seasons under two-time points (14 and 28 days). The correlation study identified a positive association between shoot dry weight and root dry weight. The genome-wide association study was conducted on root traits of 14 and 28 days with 2 million single-nucleotide polymorphisms (SNPs) using an efficient mixed model. QTLs over a significant threshold of p < 0.0001 and a 10% false discovery rate were selected to identify genes involved in root growth related to root architecture and nutrient acquisition from 97 QTLs. Candidate genes under these QTLs were explored. On chromosome 4, around 30 Mbp are two important peptide transporters (PTR5 and PTR6) involved in mobilizing nitrogen in the root during the early vegetative stage. In addition, several P transporters and expansin genes with superior haplotypes are discussed. A novel QTL from 21.12 to 21.46 Mb on chromosome 7 with two linkage disequilibrium (LD) blocks governing root length at 14 days were identified. The QTLs/candidate genes with superior haplotype for early root vigor reported here could be explored further to develop genotypes for DSR conditions.

Keywords: aus; genome-wide association study (GWAS); haplotype; nitrogen; rice; root; root vigor.