Multilocus Sequence Typing and Antifungal Susceptibility of Candida albicans Isolates From Milk and Genital Tract of Dromedary Camel

Mahmoud M Fayez; Ayman A Swelum; Nada K Alharbi; Ahlam H AlRokban; Abdullah Almubarak; Ameen H Almubarak; Fanan Alaql; Ahmed E Ahmed

doi:10.3389/fvets.2022.905962

Multilocus Sequence Typing and Antifungal Susceptibility of Candida albicans Isolates From Milk and Genital Tract of Dromedary Camel

Front Vet Sci. 2022 Jul 8:9:905962. doi: 10.3389/fvets.2022.905962. eCollection 2022.

Authors

Mahmoud M Fayez^{1

2}, Ayman A Swelum³, Nada K Alharbi⁴, Ahlam H AlRokban⁴, Abdullah Almubarak², Ameen H Almubarak², Fanan Alaql⁵, Ahmed E Ahmed^{6

7}

Affiliations

¹ Department of Bacteriology, Veterinary Serum and Vaccine Research Institute, Ministry of Agriculture, Cairo, Egypt.
² Al Ahsa Laboratory, Ministry of Agriculture Kingdom of Saudi Arabia, Al Ahsa, Saudi Arabia.
³ Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
⁴ Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
⁵ Riyadh Veterinary Diagnostic Lab, Ministry of Environment, Water and Agriculture, Riyadh, Saudi Arabia.
⁶ Biology Department, College of Science, King Khalid University, Abha, Saudi Arabia.
⁷ Department of Theriogenology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.

Abstract

Multilocus sequence typing (MLST) was used to study the genetic diversity and population structure of 48 Candida albicans (C. albicans) isolates from the udder or genital tract of apparently healthy or diseased camels. This study aimed also to determine the frequency of C. albicans isolates in the genital tract and udder of healthy or diseased female dromedary camels. A total of 240 mature dromedary camels (230 females and 10 males) were categorized based on the clinical examination of gentile tract and udder into five groups [fertile females (n = 70), infertile females (n = 115), healthy udder (n = 15), mastitis (n = 30), and fertile males (n = 10)]. Swabs were collected from male and female genital tracts of dromedary camels and milk samples were collected from healthy and diseased udders. C. albicans was isolated from 20% of the samples. The frequency of isolation was significantly higher (p < 0.00001) in disease camels (75%) compared with apparently healthy camels (25%). Most of C. albicans was isolated from infertile female genitalia (62.50%) which was significantly higher than that isolated from fertile female genitalia (16.67%). Multilocus sequence (MLS) analysis identified seven different diploid sequence types (DSTs) including DST2, DST50, DST62, DST69, DST124, DST142, and DST144. The most frequently identified DTS was DST69 (13/48) which significantly higher (p ≤ 0.05) than DST2, DST62, and DST124. The frequency of identification of DST50, DST142, and DST 144 was significantly higher (p ≤ 0.05) than DST62. DST62 and DST124 were isolated only from diseased camels. DST62 was isolated only from mastitic milk. DST124 was isolated only from infertile female genitalia. The percentage of DST50 and DST 142 was significantly higher in diseased camels (infertile females) than in the apparently healthy ones (fertile females). DST2 and DST50 were isolated only from female genitalia of apparent health and diseased camels. The C. albicans isolated from diseased camels had significantly higher biofilm formation, hydrophobicity, phospholipase, proteinase, and hemolysin activities compared with the isolates from apparent healthy camels. All isolates were sensitive to amphotericin B, itraconazole, micafungin, posaconazole and voriconazole. In conclusion, the present study represents the first molecular typing of C. albicans in samples isolated from milk and the genital tract of the dromedary camel. MLST is a useful tool for studying the epidemiology and evolution of C. albicans. Early identification of Candida species and attention to Candida virulence factors and their antifungal susceptibility patterns is very important for establishing strategies to control and/or prevent candidiasis by novel therapeutic management. Amphotericin B, itraconazole, micafungin, posaconazole, or voriconazole can be efficient in treatment of candidiasis.

Keywords: Candida; MLS; camel; fertility; infertility.