Generation of induced pluripotent stem cell line, ICGi033-A, by reprogramming peripheral blood mononuclear cells from a patient with Huntington's disease

Elena V Grigor'eva; Anastasia A Malakhova; Diana A Sorogina; Sofia V Pavlova; Tuyana B Malankhanova; Natalia Yu Abramycheva; Sergey A Klyushnikov; Sergey N Illarioshkin; Suren M Zakian

doi:10.1016/j.scr.2022.102868

Generation of induced pluripotent stem cell line, ICGi033-A, by reprogramming peripheral blood mononuclear cells from a patient with Huntington's disease

Stem Cell Res. 2022 Aug:63:102868. doi: 10.1016/j.scr.2022.102868. Epub 2022 Jul 13.

Authors

Elena V Grigor'eva¹, Anastasia A Malakhova², Diana A Sorogina³, Sofia V Pavlova², Tuyana B Malankhanova³, Natalia Yu Abramycheva⁴, Sergey A Klyushnikov⁴, Sergey N Illarioshkin⁴, Suren M Zakian²

Affiliations

¹ Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia; E. Meshalkin National Medical Research Center of the Ministry of Health of the Russian Federation, Novosibirsk, Russia; Institute of Chemical Biology and Fundamental Medicine, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia. Electronic address: evlena@bionet.nsc.ru.
² Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia; E. Meshalkin National Medical Research Center of the Ministry of Health of the Russian Federation, Novosibirsk, Russia; Institute of Chemical Biology and Fundamental Medicine, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
³ Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
⁴ Research Center of Neurology, Moscow, Russia.

PMID: 35872525
DOI: 10.1016/j.scr.2022.102868

Abstract

Huntington's disease (HD) is a hereditary autosomal dominant neurodegenerative disease caused by the polyglutamine stretch expansion in the huntingtin (HTT) protein. In HD, dysregulation of multiple cellular processes occurs, resulting in the death of medium spiny neurons of striatum. A line of induced pluripotent stem cells (iPSCs) ICGi033-A was obtained from peripheral blood mononuclear cells of a patient carrying 77 CAG repeats in the HTT gene. The iPSCs express pluripotency markers, have a normal karyotype, and differentiate into three germ layers: endoderm, ectoderm, mesoderm.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Humans
Huntingtin Protein / genetics
Huntington Disease* / genetics
Huntington Disease* / metabolism
Induced Pluripotent Stem Cells* / metabolism
Leukocytes, Mononuclear / metabolism
Neurodegenerative Diseases* / metabolism

Substances

Huntingtin Protein