Carotenoid extraction and analysis from blood plasma/serum

Maria J Sholola; Jessica L Cooperstone

doi:10.1016/bs.mie.2022.03.021

Carotenoid extraction and analysis from blood plasma/serum

Methods Enzymol. 2022:670:423-457. doi: 10.1016/bs.mie.2022.03.021. Epub 2022 Apr 11.

Authors

Maria J Sholola¹, Jessica L Cooperstone²

Affiliations

¹ Food Science and Technology, The Ohio State University, Columbus, OH, United States.
² Food Science and Technology, The Ohio State University, Columbus, OH, United States; Horticulture and Crop Science, The Ohio State University, Columbus, OH, United States. Electronic address: cooperstone.1@osu.edu.

PMID: 35871843
DOI: 10.1016/bs.mie.2022.03.021

Abstract

Accurate and sensitive methods to quantify carotenoids in blood plasma/serum are the basis for assessing carotenoid intake and associating physiological effects. This chapter introduces carotenoid chemistry, an overview of carotenoid food sources, and current knowledge of carotenoid absorption and metabolism, along with factors that affect these processes. We also detail a commonly used method to extract plasma/serum carotenoids using liquid-liquid extraction and analysis by high performance liquid chromatography coupled with diode array detection (HPLC-DAD). A spreadsheet to aid in this quantitative analysis can be found at www.github.com/CooperstoneLab/carotenoid-analysis.

Keywords: Blood; Carotenoids; HPLC; Liquid-liquid extraction; Plasma; Quantitative analysis; Serum.

MeSH terms

Carotenoids* / metabolism
Chromatography, High Pressure Liquid / methods
Plasma* / chemistry
Plasma* / metabolism

Substances

Carotenoids