Protein cross-linking plays important roles in food, chemical, medicine and other fields. Enzyme-catalyzed protein cross-linking is an efficient and economically viable alternative to physical and chemical cross-linking. However, detailed analysis of enzyme-catalyzed protein cross-linking at molecular level is still lacking. This review summarized the mechanisms of enzyme-catalyzed protein cross-linking, its effects on protein structure, and its applications in food, chemical and pharmaceutical fields.
Keywords: glutamyl endoproteinase; industrial application; lipoxygenase; oxidase; protein cross-linking; transglutaminase.