Association of OPG and RANKL gene polymorphisms with bone mineral density in Indian women

Sandhya Nair; Hetal Bhadricha; Anushree Patil; Suchitra Surve; Beena Joshi; Nafisa Balasinor; Meena Desai

doi:10.1016/j.gene.2022.146746

Association of OPG and RANKL gene polymorphisms with bone mineral density in Indian women

Gene. 2022 Oct 5:840:146746. doi: 10.1016/j.gene.2022.146746. Epub 2022 Jul 20.

Authors

Sandhya Nair¹, Hetal Bhadricha¹, Anushree Patil², Suchitra Surve², Beena Joshi³, Nafisa Balasinor⁴, Meena Desai⁵

Affiliations

¹ Molecular Immunodiagnostics Division, ICMR-National Institute for Research in Reproductive and Child Health, Jehangir Merwanji Street, Parel, Mumbai 400 012, India.
² Department of Clinical Research, ICMR-National Institute for Research in Reproductive and Child Health, Jehangir Merwanji Street, Parel, Mumbai 400 012, India.
³ Department of Operational Research, ICMR-National Institute for Research in Reproductive and Child Health, Jehangir Merwanji Street, Parel, Mumbai 400 012, India.
⁴ Neuroendocrinology Division, ICMR-National Institute for Research in Reproductive and Child Health, Jehangir Merwanji Street, Parel, Mumbai 400 012, India.
⁵ Molecular Immunodiagnostics Division, ICMR-National Institute for Research in Reproductive and Child Health, Jehangir Merwanji Street, Parel, Mumbai 400 012, India. Electronic address: desaim@nirrh.res.in.

PMID: 35868414
DOI: 10.1016/j.gene.2022.146746

Abstract

Osteoprotegerin (OPG) and receptor activator of the NF-kB ligand (RANKL) are key players in bone remodelling. Reports show that OPG and RANKL gene polymorphisms are associated with osteoporosis and fracture risk. The aim of this study was to examine the influence of 5 single nucleotide polymorphisms (SNPs) in OPG and RANKL gene on bone mineral density (BMD) in Indian women. The study included 374 healthy Indian women. Kompetitive Allele Specific PCR (KASP) was used for genotyping. There was a significant difference in the BMD at spine between genotypes of OPG rs2073618 (CC: 0.988 ± 0.167 CG: 1.023 ± 0.17 GG: 1.053 ± 0.155; p = 0.039) which was lost upon adjustment for age and BMI (p = 0.087). Multiple linear regression revealed that genotypes of OPG rs2073618 (β = 0.098; p = 0.027) and rs3102735 (β = 0.092; p = 0.038) are predictors of BMD at spine in Indian women. We did not observe any association of SNPs in RANKL gene with BMD. Thus, SNPs rs2073618 and rs3102735 in OPG gene may influence BMD at spine in Indian women.

Keywords: BMD; OPG; Polymorphisms; RANKL.

MeSH terms

Bone Density* / genetics
Female
Humans
Ligands
Osteoprotegerin / genetics*
Polymorphism, Single Nucleotide
RANK Ligand / genetics*

Substances

Ligands
Osteoprotegerin
RANK Ligand
TNFRSF11B protein, human
TNFSF11 protein, human