Exosomal microRNA expression profiles derived from A549 human lung cells in response to influenza A/H1N1pdm09 infection

Yiyue Ge; Kang Liu; Ying Chi; Xiaojuan Zhu; Tao Wu; Kangchen Zhao; Qiao Qiao; Bin Wu; Fengcai Zhu; Lunbiao Cui

doi:10.1016/j.virol.2022.07.009

Exosomal microRNA expression profiles derived from A549 human lung cells in response to influenza A/H1N1pdm09 infection

Virology. 2022 Sep:574:9-17. doi: 10.1016/j.virol.2022.07.009. Epub 2022 Jul 16.

Authors

Yiyue Ge¹, Kang Liu², Ying Chi³, Xiaojuan Zhu³, Tao Wu³, Kangchen Zhao³, Qiao Qiao³, Bin Wu³, Fengcai Zhu³, Lunbiao Cui⁴

Affiliations

¹ Institute of Pathogenic Microbiology, NHC Key Laboratories of Enteric Pathogenic Microbiology, Jiangsu Province Engineering Research Center of Health Emergency, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China. Electronic address: geyiyue@163.com.
² College of Pharmacy, Nankai University, Tianjin, 300071, China.
³ Institute of Pathogenic Microbiology, NHC Key Laboratories of Enteric Pathogenic Microbiology, Jiangsu Province Engineering Research Center of Health Emergency, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
⁴ Institute of Pathogenic Microbiology, NHC Key Laboratories of Enteric Pathogenic Microbiology, Jiangsu Province Engineering Research Center of Health Emergency, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China. Electronic address: lbcui@jscdc.cn.

PMID: 35868217
DOI: 10.1016/j.virol.2022.07.009

Abstract

Exosomes participate in intercellular communication by shuttling various small molecules from donor to recipient cells. We aimed to examine the role of exosomes and exosomal miRNAs in influenza virus infection. The results showed that influenza A/H1N1pdm09 infection could promote A549 cells to secrete exosomes, while blocking the generation of exosomes reduced viral RNA production. A total of 97 exosomal miRNAs with significantly altered expression were identified during influenza infection. Of 12 candidate miRNAs chosen for further validation, ten were confirmed by qRT-PCR. Among 5978 predicted target genes，we found 37 interferon pathway-related genes to be the potential targets of 29 differentially expressed miRNAs. Many target genes were annotated to various KEGG signaling pathways, some of which played important roles in influenza infection. These data will help to further understand the mechanism of influenza virus-host interactions, which is important for the development of preventative and therapeutic strategies against influenza virus.

Keywords: A/H1N1pdm09; A549 cell; Exosomal microRNA; Influenza; Profile.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

A549 Cells
Exosomes* / genetics
Exosomes* / metabolism
Humans
Influenza, Human* / genetics
Influenza, Human* / metabolism
Lung / metabolism
MicroRNAs* / genetics
MicroRNAs* / metabolism

Substances

MicroRNAs