Structural features of free N-glycans in α1,3/4-fucosidase-deficient Arabidopsis thaliana: deletion of α1,3/4-fucosidase activity induced accumulation of plant complex type GN1 free N-glycans

Shun Takata; Megumi Hayashi; Megumi Maeda; Takeshi Ishimizu; Yoshinobu Kimura

doi:10.1093/bbb/zbac120

Structural features of free N-glycans in α1,3/4-fucosidase-deficient Arabidopsis thaliana: deletion of α1,3/4-fucosidase activity induced accumulation of plant complex type GN1 free N-glycans

Biosci Biotechnol Biochem. 2022 Sep 23;86(10):1413-1416. doi: 10.1093/bbb/zbac120.

Authors

Shun Takata¹, Megumi Hayashi², Megumi Maeda^{1

3}, Takeshi Ishimizu², Yoshinobu Kimura^{1

3}

Affiliations

¹ Faculty of Agriculture, Division of Agricultural Science, Okayama University, 1-1-1 Tsushima-Naka, Kita-ku, Okayama, Japan.
² College of Life Sciences, Ritsumeikan University, Kusatsu, Shiga, Japan.
³ Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, Japan.

PMID: 35867865
DOI: 10.1093/bbb/zbac120

Abstract

Deletion of α-1,3/4-fucosidase activity in Arabidopsis thaliana resulted in the accumulation of GN1-type free N-glycans with the Lewis a epitope (GN1-FNG). This suggests that the release of α-fucose residue(s) may trigger rapid degradation of the plant complex-type (PCT) GN1-FNG. The fact that PCT-GN1-FNG has rarely been detected to date is probably due to its easier degradation compared with PCT-GN2-FNG.

Keywords: Arabidopsis thaliana; endo-β-N-acetylglucosaminidase; free N-glycans; peptide: N-glycanase; α-fucosidase.

MeSH terms

Arabidopsis* / genetics
Arabidopsis* / metabolism
Epitopes
Fucose / chemistry
Polysaccharides / metabolism
alpha-L-Fucosidase* / genetics
alpha-L-Fucosidase* / metabolism

Substances

Epitopes
Polysaccharides
Fucose
alpha-L-Fucosidase

Grants and funding

JP18K05559/JSPS