NDM-35-Producing ST167 Escherichia coli Highly Resistant to β-Lactams Including Cefiderocol

Laurent Poirel; José Manuel Ortiz de la Rosa; Zeynep Sakaoglu; Ayda Kusaksizoglu; Mustafa Sadek; Patrice Nordmann

doi:10.1128/aac.00311-22

NDM-35-Producing ST167 Escherichia coli Highly Resistant to β-Lactams Including Cefiderocol

Antimicrob Agents Chemother. 2022 Aug 16;66(8):e0031122. doi: 10.1128/aac.00311-22. Epub 2022 Jul 11.

Authors

Laurent Poirel^{1

2

3}, José Manuel Ortiz de la Rosa^{1

2}, Zeynep Sakaoglu¹, Ayda Kusaksizoglu¹, Mustafa Sadek^{1

2}, Patrice Nordmann^{1

2

3

4}

Affiliations

¹ Emerging Antibiotic Resistance, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerlandgrid.8534.a.
² Swiss National Reference Center for Emerging Antibiotic Resistance, Fribourg, Switzerland.
³ INSERM European Unit (LEA), (IAME, Paris), University of Fribourggrid.8534.a, Fribourg, Switzerland.
⁴ Institute for Microbiology, Lausanne University hospital and University of Lausanne, Lausanne, Switzerland.

Abstract

A multidrug-resistant (carbapenems, aztreonam + avibactam, and cefiderocol) ST167 Escherichia coli clinical isolate recovered from a patient hospitalized in Switzerland produced NDM-35 showing ca. 10-fold increased hydrolytic activity toward cefiderocol compared to NDM-1. The isolate co-produced a CMY-type β-lactamase, exhibited a four amino-acid insertion in PBP3, and possessed a truncated iron transporter CirA protein. Our study identified an association of unrelated resistance mechanisms leading to resistance to virtually all β-lactams in a high-risk E. coli clone.

Keywords: NDM; aztreonam-avibactam; cefiderocol; metallo-β-lactamase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Cefiderocol
Cephalosporins
Drug Resistance, Multiple, Bacterial / genetics
Escherichia coli Infections* / drug therapy
Escherichia coli* / genetics
Escherichia coli* / metabolism
Humans
Microbial Sensitivity Tests
beta-Lactamases / genetics
beta-Lactamases / metabolism
beta-Lactams / pharmacology

Substances

Anti-Bacterial Agents
Cephalosporins
beta-Lactams
beta-Lactamases