Recent works indicate that, at specific loci, interactions of chromatin with membrane-less organelles self-assembled through mechanisms of phase separation, like nuclear bodies, are crucial to regulate genome functions, and in particular transcription. Here we describe the protocol of the high-salt recovered sequence sequencing method whose principle relies on high-throughput sequencing of genomic DNA trapped into large RNP complexes that are made insoluble by high-salt treatments.
Keywords: High-order chromatin organization; Nuclear bodies; Quantitative PCR.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.