Quantitative Chromosome Conformation Capture (3C-qPCR)

Cosette Rebouissou; Séphora Sallis; Thierry Forné

doi:10.1007/978-1-0716-2497-5_1

Quantitative Chromosome Conformation Capture (3C-qPCR)

Methods Mol Biol. 2022:2532:3-13. doi: 10.1007/978-1-0716-2497-5_1.

Authors

Cosette Rebouissou¹, Séphora Sallis^{1

2}, Thierry Forné³

Affiliations

¹ Institut de Génétique Moléculaire de Montpellier (IGMM), University of Montpellier, CNRS, Montpellier, France.
² Laboratoire de génétique moléculaire du développement, Département des sciences biologiques, Université du Québec à Montréal, Montréal, QC, Canada.
³ Institut de Génétique Moléculaire de Montpellier (IGMM), University of Montpellier, CNRS, Montpellier, France. forne@igmm.cnrs.fr.

PMID: 35867242
DOI: 10.1007/978-1-0716-2497-5_1

Abstract

Many population-based methods investigating chromatin dynamics and organization in eukaryotes are based on the chromosome conformation capture (3C) method. Here, we provide an updated version of the quantitative 3C (3C-qPCR) protocol for improved and simplified quantitative analyses of intra-chromosomal contacts.

Keywords: Chromatin dynamics and organization; Chromosome conformation capture; Eukaryotic genome; Interaction frequency; Quantitative PCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatin* / genetics
Chromosomes* / genetics
Molecular Conformation
Nucleic Acid Conformation
Real-Time Polymerase Chain Reaction

Substances

Chromatin