Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP

Zhenhao Zhang; Tong He; Yan Qi; Yuxuan Dai; Kejing Lao; Xingchun Gou

doi:10.1039/d2ra03310k

Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP

RSC Adv. 2022 Jul 7;12(31):19885-19889. doi: 10.1039/d2ra03310k. eCollection 2022 Jul 6.

Authors

Zhenhao Zhang^{1

2}, Tong He^{1

2}, Yan Qi¹, Yuxuan Dai¹, Kejing Lao¹, Xingchun Gou¹

Affiliations

¹ Shaanxi Key Laboratory of Brain Disorders, Shaanxi Provincial Key Laboratory of Infection and Immunity Diseases, Institute of Basic and Translational Medicine, Xi'an Medical University No. 1 Xin Wang Road Xi'an 710021 China gouxingchun@189.cn +86 29 86177603.
² School of Chemistry and Chemical Engineering, Shaanxi Normal University Xi'an 710062 Shaanxi Province P. R. China.

Abstract

We have developed a rapid and specific method for site-specific 5-hydroxymethylcytosine (5hmC) quantification at single-base resolution. This bisulfite-free method integrates the peroxotungstate oxidization with the mismatched probe-assisted ligation to guarantee the specificity. Moreover, the high-efficiency LAMP also makes the proposed method suitable for the detection of low-content samples.