Myelin like electrogenic filamentation and Liquid Microbial Fuel Cells Dataset

Emilio D'Ugo; Lucia Bertuccini; Francesca Spadaro; Roberto Giuseppetti; Francesca Iosi; Fabio Santavenere; Fausto Giuliani; Milena Bruno; Nicola Lovecchio; Silvia Gioacchini; Paola Bucci; Emilia Stellacci; Antonietta Bernardo; Arghya Mukherjee; Fabio Magurano

doi:10.1016/j.dib.2022.108447

Myelin like electrogenic filamentation and Liquid Microbial Fuel Cells Dataset

Data Brief. 2022 Jul 11:43:108447. doi: 10.1016/j.dib.2022.108447. eCollection 2022 Aug.

Authors

Affiliations

¹ Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy.
² Core Facilities, Istituto Superiore di Sanità, Rome, Italy.
³ National Center for Innovative Technologies in Public Health, Istituto Superiore di Sanità, Rome, Italy.
⁴ Department of Information Engineering, Electronics and Telecommunications, Sapienza, University of Rome, Rome, Italy.
⁵ Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy.
⁶ National Center for Research and Preclinical and Clinical Evaluation of Drugs, Istituto Superiore di Sanità, Rome, Italy.
⁷ Department of Food Biosciences, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland.

Abstract

Biofilm at water-oil interface of hypoxic water columns of microcosms, prepared from a lacustrine sample, that used diesel as a carbon source was found to show electrogenic properties. These microcosms named, Liquid Microbial Fuel Cells (L-MFCs) were electrically characterized using a custom electronic analyzer; accurate determination of voltage (V), power density (W/m 2), and current density (A/m2) for both charge and discharge phases was carried out. The instrument made it possible to carry out cell characterizations using resistive loads between 0 Ω (Ohm) and 10 kΩ. During the hypoxic and electrogenic phase, the synthesis of a system of "bacterial piping induction", produced filaments of hundreds of micrometers in which the microbial cells are hosted. Ultrastructural microscopy collected by scanning (SEM), transmission (TEM), immunofluorescence, Thunder Imager 3D, confocal laser scanning (CLSM) microscopy revealed a "myelin like" structure during filamentation processes; this "myelin like" structure exhibited cross-reactivity towards different epitopes of the myelin basic protein (MBP) and Claudin 11 (O4) of human oligodendrocytes. The disclosure of these filamentation processes could be helpful to describe further unconventional microbial structures in aquatic ecosystems and of the animal world. The data that support the findings of this study are openly available in at https://data.mendeley.com/datasets/7d35tj3j96/1.

Keywords: 16S, ribosomal gene; : L-MFCs, liquid microbial fuel cells; A/m2, current density; ABS, acrylonitrile-butadiene-styrene; CLSM, confocal laser scanning microscopy; DAPI dye, 2-[4-(aminoiminomethyl)phenyl]-1H-indole-6-carboximidamide hydrochloride; Electrogenic biofilm; FM 1-43 dye, N-3-triethylammoniumpropyl-4-4-dibutylamino styryl pyridinium dibromide; Filamentation; HMDS, hexamethyldisilazane; Hydrocarbonoclastic biofilm; LB, Luria-Bertani broth; M9, medium; MBP, myelin basic protein; Microbial evolution; Microbial fuel cells; Myelin basic protein; Myelin sheath; Myelin-like filaments; O4, claudin 11; OD, optical density; PCR, polymerase chain reaction; PMMA, polymethylmethacrylate; PVC, polyvinylchloride; RT, room temperature; Rp, product resistance; SEM, scanning electron microscopy; SEM, scanning microscopy; SOP, standard operating procedure; SRA, sequence read archive; TEM, transmission; TEM, transmission electron microscopy; V, voltage; W/m 2, power density; W/m2, watts per meter square (power density); rRNA, ribosomal ribonucleic acid; Ω, Ohm.

Grants and funding

DP2 AI138259/AI/NIAID NIH HHS/United States