Electrical voltage application as a novel approach for facilitating methanogenic granulation

Abstract

Despite having high-rate methanogenic performance, up-flow anaerobic sludge blanket reactor still has challenges regarding long-start up period (3-8 months) for granulation. In this study, "electrical voltage (EV, 0.3 V) application" was attempted for facilitating granulation in the continuous operation with increased organic loading rates (0.5-11.0 kg COD/m³/d). Up to 11.0 kg COD/m³/d, EV-reactor exhibited the stable performance, while the control failed. After 49 days of operation (at 7 kg COD/m³/d), the granules collected from EV-reactor had larger diameter (2.3 vs 1.6 mm), higher settling velocity (2.6 vs 1.9 cm/s), and higher hydrophobicity (52.1 % vs 34.5 %), compared to the control. EV application also increased the specific methanogenic activity for propionate and hydrogen almost by two times. The relative abundance of Pseudomonas sp. (quorum sensing (QS)-related microbe) in EV-reactor was 17 % higher than that in the control. In addition, EV application increased the expression of QS genes significantly by 27 times.

Keywords: Direct interspecies electron transfer (DIET); Electrical voltage; Granulation; Physico-chemical characteristics; Quorum sensing.