Investigating Pathways of Ventilation Induced Brain Injury on Cerebral White Matter Inflammation and Injury After 24 h in Preterm Lambs

Kyra Yy Chan; Nhi T Tran; Paris C Papagianis; Valerie A Zahra; Ilias Nitsos; Alison M Moxham; Domenic A LaRosa; Courtney McDonald; Suzanne L Miller; Robert Galinsky; Dhafer M Alahmari; Vanesa Stojanovska; Graeme R Polglase

doi:10.3389/fphys.2022.904144

Investigating Pathways of Ventilation Induced Brain Injury on Cerebral White Matter Inflammation and Injury After 24 h in Preterm Lambs

Front Physiol. 2022 Jul 4:13:904144. doi: 10.3389/fphys.2022.904144. eCollection 2022.

Authors

Kyra Yy Chan^{1

2}, Nhi T Tran^{1

2}, Paris C Papagianis¹, Valerie A Zahra¹, Ilias Nitsos^{1

2}, Alison M Moxham¹, Domenic A LaRosa^{1

2}, Courtney McDonald^{1

2}, Suzanne L Miller^{1

2}, Robert Galinsky^{1

2}, Dhafer M Alahmari^{3

4

5}, Vanesa Stojanovska^{1

2}, Graeme R Polglase^{1

2}

Affiliations

¹ The Ritchie Centre, Hudson Institute of Medical Research, Clayton, VIC, Australia.
² Department of Obstetrics and Gynaecology, Monash University, Clayton, VIC, Australia.
³ Monash Biomedicine Discovery Institute and Department of Medical Imaging and Radiation Sciences, Monash University, Clayton, VIC, Australia.
⁴ Monash Biomedical Imaging, Monash University, Clayton, VIC, Australia.
⁵ Department of Diagnostic Imaging, King Saud Medical City, Riyadh, Saudi Arabia.

Abstract

Initiation of respiratory support in the delivery room increases the risk and severity of brain injury in preterm neonates through two major pathways: an inflammatory pathway and a haemodynamic pathway. The relative contribution of each pathway on preterm brain injury is not known. We aimed to assess the role of the inflammatory and haemodynamic pathway on ventilation-induced brain injury (VIBI) in the preterm lamb. Fetal lambs (125 ± 1 day gestation) were exteriorised, instrumented and ventilated with a high tidal-volume (V_T) injurious strategy for 15 min either with placental circulation intact to induce the inflammatory pathway only (INJ_INF; n = 7) or umbilical cord occluded to induce both the inflammatory and haemodynamic pathways (INJ_INF+HAE; n = 7). Sham controls were exteriorised but not ventilated (SHAM; n = 5) while unoperated controls (UNOP; n = 7) did not undergo fetal instrumentation. Fetuses were returned in utero following intervention and the ewe allowed to recover. Arterial blood gases and plasma were sampled periodically. Twenty-four hours following intervention, lambs were delivered and maintained on non-injurious ventilation for ∼40 min then brains were collected post-mortem for immunohistochemistry and RT-qPCR to assess inflammation, vascular pathology and cell death within white matter regions. Compared to INJ_INF lambs, INJ_INF+HAE lambs achieved a consistently higher V_T during injurious ventilation and carotid blood flow was significantly lower than baseline by the end of ventilation. Throughout the 24 h recovery period, systemic arterial IL-6 levels of INJ_INF+HAE lambs were significantly higher than SHAM while there was no difference between INJ_INF and SHAM animals. At 24 h, mRNA expression levels of pro-inflammatory cytokines, tight junction proteins, markers of cell death, and histological injury indices of gliosis, blood vessel protein extravasation, oligodendrocyte injury and cell death were not different between groups. Injurious ventilation, irrespective of strategy, did not increase brain inflammation or injury 24 h later when compared to control animals. However, the haemodynamic pathway did influence carotid blood flow adaptations during injurious ventilation and increased systemic arterial IL-6 that may underlie long-term pathology. Future studies are required to further characterise the pathways and their long-term effects on VIBI.

Keywords: cerebral inflammation; mechanical ventilation; mechanisms; preterm; ventilation-induced brain injury; white matter injury.