Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments

V A Korolenya; K A Gavrilov; K S Sevost'ianova; A I Shevela; M L Filipenko; M A Smetanina

doi:10.1007/s10517-022-05554-8

Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments

Bull Exp Biol Med. 2022 Jul;173(3):384-389. doi: 10.1007/s10517-022-05554-8. Epub 2022 Jul 19.

Authors

V A Korolenya^{1

2}, K A Gavrilov^{1

2}, K S Sevost'ianova^{1

2}, A I Shevela^{1

2}, M L Filipenko^{1

2}, M A Smetanina^{3

4}

Affiliations

¹ Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
² Novosibirsk National Research State University, Novosibirsk, Russia.
³ Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. mariya_smetanina@niboch.nsc.ru.
⁴ Novosibirsk National Research State University, Novosibirsk, Russia. mariya_smetanina@niboch.nsc.ru.

PMID: 35852690
DOI: 10.1007/s10517-022-05554-8

Abstract

Contamination of the sample with DNA is a problem when isolating RNA by phenol-chloroform extraction, and DNase treatment introduces an additional error in the analysis of gene expression. Bearing in mind the ability of LiCl to precipitate RNA selectively, we studied the possibility and advantage of using LiCl as a precipitation agent in the protocol for RNA precipitation [3] from frozen segments of large vessels. Combined use of LiCl with isopropanol as precipitating agents (optimal ratio 2.5 M and 40%, respectively) significantly eliminates negative effects of using this salt (inhibition of reverse transcription and low RNA yield).

Keywords: RNA isolation; isopropanol; lithium chloride; precipitation selectivity; reverse transcription.

MeSH terms

DNA
Lithium
Lithium Chloride* / pharmacology
Phenol
RNA* / genetics

Substances

Phenol
RNA
DNA
Lithium
Lithium Chloride