A Ratiometric Photoacoustic Probe with a Reversible Response to Hydrogen Sulfide and Hydroxyl Radicals for Dynamic Imaging of Liver Inflammation

Abstract

Reversible imaging probes that allow for the dynamic visualization of the redox cycle between hydroxyl radical (⋅OH) and hydrogen sulfide (H₂ S) are vital to probe the redox imbalance-involved pathological process in vivo. Herein, we report a reversible ratiometric photoacoustic (PA) imaging nanoprobe (1-PAIN) for the real-time imaging of ⋅OH/H₂ S redox cycle in vivo. 1-PAIN displays a low PA ratio between 690 and 825 nm (PA₆₉₀ /PA₈₂₅ ), which significantly increases by ≈5-fold upon oxidation by ⋅OH, and is switched back to the initially low PA₆₉₀ /PA₈₂₅ value upon reduction by H₂ S. 1-PAIN could dynamically report on the hepatic ⋅OH production in mice during the lipopolysaccharide (LPS)-induced liver inflammation process, and visualize hepatic H₂ S generation during the N-acetyl cysteine (NAC)-induced anti-inflammation process. 1-PAIN can act as a useful tool to probe the redox state in living biology, beneficial for the study of redox imbalance-related diseases.

Keywords: Hydrogen Sulfide; Hydroxyl Radical; Photoacoustic Imaging; Ratiometric Imaging; Reversible Probe.