Involvement of PtCOL5-PtNF-YC4 in reproductive cone development and gibberellin signaling in Chinese pine

Hongmei Liu; Yingtian Guo; Huili Wang; Wenbin Yang; Junhe Yang; Jingxing Zhang; Dan Liu; Yousry A El-Kassaby; Wei Li

doi:10.1016/j.plantsci.2022.111383

Involvement of PtCOL5-PtNF-YC4 in reproductive cone development and gibberellin signaling in Chinese pine

Plant Sci. 2022 Oct:323:111383. doi: 10.1016/j.plantsci.2022.111383. Epub 2022 Jul 16.

Authors

Hongmei Liu¹, Yingtian Guo², Huili Wang³, Wenbin Yang⁴, Junhe Yang⁵, Jingxing Zhang⁶, Dan Liu⁷, Yousry A El-Kassaby⁸, Wei Li⁹

Affiliations

¹ National Engineering Laboratory of Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China. Electronic address: liuhongmei6717@163.com.
² National Engineering Laboratory of Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China. Electronic address: gytxiaozi@163.com.
³ National Engineering Laboratory of Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China. Electronic address: wanghuili1997@163.com.
⁴ National Engineering Laboratory of Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China. Electronic address: bjfuywb@bjfu.edu.cn.
⁵ National Engineering Laboratory of Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China. Electronic address: 1092877903@qq.com.
⁶ National Engineering Laboratory of Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China. Electronic address: 199393790@qq.com.
⁷ National Engineering Laboratory of Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China. Electronic address: 1821618@163.com.
⁸ Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, 2424 Main Mall, Vancouver, BC V6T 1Z4, Canada. Electronic address: y.el-kassaby@ubc.ca.
⁹ National Engineering Laboratory of Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China. Electronic address: bjfuliwei@bjfu.edu.cn.

PMID: 35850285
DOI: 10.1016/j.plantsci.2022.111383

Abstract

It is well documented that the CO/NF-YB/NF-YC trimer (NF-Y-CO) binds and regulates the FT promoter. However, the FT/TFL1-like (FLOWERING LOCUS T/TERMINALFLOWER1-like) genes in gymnosperms are all flowering suppressors, and the regulation model of NF-Y in gymnosperms is different from that in angiosperms. Here, using Chinese pine (Pinus tabuliformis), we identified a CONSTANS-LIKE gene, PtCOL5, the expression of which was strongly induced during cones development and it functioned as a repressor of flowering. PtNF-YC4, which interacted with PtCOL5, was highly correlated with PtCOL5 during growth and development, has been demonstrated. Moreover, PtNF-YC4 and PtCOL5 can bind to PtTFL2 promoter, and their interaction can enhance PtTFL2 expression. Interestingly, we found PtNF-YC4 and PtCOL5 were involved in gibberellin signaling and their interaction was inhibited by PtDELLA protein, thus affecting PtTFL2 expression. Collectively, PtCOL5-PtNF-YC4 was involved in reproductive cone development and gibberellin signaling in Chinese pine. Our findings uncovered reproductive cone development and signal transduction mechanism of COL-NF-Y in gymnosperms.

Keywords: Chinese pine; PtCOL5; PtDELLA; PtNF-YC4; PtTFL2; Reproductive cone development.

MeSH terms

China
Flowers / genetics
Gene Expression Regulation, Plant*
Gibberellins
Pinus* / genetics
Pinus* / metabolism
Plant Proteins / genetics
Plant Proteins / metabolism
Signal Transduction

Substances

Gibberellins
Plant Proteins