Nano artificial periosteum PLGA/MgO/Quercetin accelerates repair of bone defects through promoting osteogenic - angiogenic coupling effect via Wnt/ β-catenin pathway

Xi He; Wenbin Liu; Yanling Liu; Kai Zhang; Yan Sun; Pengfei Lei; Yihe Hu

doi:10.1016/j.mtbio.2022.100348

Nano artificial periosteum PLGA/MgO/Quercetin accelerates repair of bone defects through promoting osteogenic - angiogenic coupling effect via Wnt/ β-catenin pathway

Mater Today Bio. 2022 Jul 1:16:100348. doi: 10.1016/j.mtbio.2022.100348. eCollection 2022 Dec.

Authors

Xi He^{1

2}, Wenbin Liu^{2

3}, Yanling Liu⁴, Kai Zhang^{2

3}, Yan Sun^{2

3}, Pengfei Lei^{2

3

5}, Yihe Hu^{1

2

3

5}

Affiliations

¹ Department of Sports Medicine, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China.
² Hunan Engineering Research Center of Biomedical Metal and Ceramic Implants, Changsha, China.
³ Department of Orthopedic Surgery, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China.
⁴ Provincial Laboratory for Diagnosis and Treatment of Genitourinary System Disease, Department of Urology, Xiangya Hospital, Central South University, Changsha, China.
⁵ Department of Orthopedics, The First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, China.

Abstract

Bone nonunion or delayed union, caused by stripping or injuring of periosteum, is the most common sequelae of segmental bone defects. The preservation of periosteum, or the use of periosteal grafts, can significantly improve the integration of bone graft, speeding up the process of bone reconstruction. However, in most cases, periosteum cannot be preserved with bioactivity. Thus, it is pivotal to develop artificial periosteum. In this study, artificial periosteum of PLGA/MgO/Quercetin was prepared by electrospinning. PLGA/MgO/Quercetin membranes were shown to have a highly porous surface and microstructure, as observed by scanning electron microscopy. Along with excellent biocompatibility, PLGA/MgO/Quercetin membranes promoted cell proliferation and migration, as well as osteogenic differentiation of BMSCs (Bone marrow mesenchymal stem cells) in a dose-dependent manner through the activation of Wnt/β-Catenin pathway. The PLGA/MgO/Quercetin membranes, with an appropriate concentration of quercetin (<1 wt%), promoted EPCs (Endothelial progenitor cells) angiogenesis. In a subcutaneous implantation model and rat skull defect model, optimal osteogenesis and angiogenesis function were observed for the PLGA/20 wt% MgO/0.1 wt% Quercetin membrane. In conclusion, PLGA/MgO membranes, with an appropriate concentration of quercetin, show a variety of biological activities and are promising materials for the generation of artificial periosteum.

Keywords: Angiogenesis; Artificial periosteum; Electrospinning; Osteogenesis; Quercetin.