Inhibitory Effect of Fermented Flammulina velutipes Polysaccharides on Mice Intestinal Inflammation

Sheng Ma; Jianxiong Xu; Ting Lai; Weina Xu; Jing Zhang; Hongcai Zhang; Weiyi Zhang

doi:10.3389/fnut.2022.934073

Inhibitory Effect of Fermented Flammulina velutipes Polysaccharides on Mice Intestinal Inflammation

Front Nutr. 2022 Jun 21:9:934073. doi: 10.3389/fnut.2022.934073. eCollection 2022.

Authors

Sheng Ma^{1

2}, Jianxiong Xu^{1

2}, Ting Lai^{1

2}, Weina Xu^{1

2}, Jing Zhang^{1

2}, Hongcai Zhang^{1

2}, Weiyi Zhang³

Affiliations

¹ School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
² Shanghai Key Laboratory for Veterinary and Biotechnology, Shanghai, China.
³ Shanghai Center of Agri-Products Quality and Safety, Shanghai, China.

Abstract

To investigate the effect of Flammulina velutipes polysaccharides (FVPs) on mice intestinal inflammation, FVPs were extracted from Flammulina velutipes (FV) using a solid anaerobic fermentation technique. The antioxidant and anti-inflammatory capacities of FVP and fermented FVP (FFVP) induced by lipopolysaccharide (LPS) were investigated in vitro and in vivo. The results showed that the yield of FFVP (9.44%) was higher than that of FVP (8.65%), but the molecular weight (MW) of FFVP (15,702 Da) was lower than that of FVP (15,961 Da). The antioxidant and anti-inflammatory capacities of FFVP were higher than that of FVP in preventing mice diarrhea, enhancing antioxidant capacities, and reducing the secretion and mRNA expression of interleukin-1β (IL-1β), IL-6, IL-18, and tumor necrosis factor-α (TNF-α). The anti-inflammatory mechanisms of FVP and FFVP were analyzed by inhibiting the activation of the NLRP3 signaling pathway using an LPS-induced mice model. This study indicated that FFVP could be used as a functional antioxidant, indicating a potential application in functional food and health products.

Keywords: Flammulina velutipes polysaccharides; NLRP3 signaling pathway; anti-inflammatory capacities; antioxidant capacities; solid anaerobic fermentation.