The Physical Location of Stripe Rust Resistance Genes on Chromosome 6 of Rye (Secale cereale L.) AR106BONE

Yanling Duan; Jie Luo; Zujun Yang; Guangrong Li; Zongxiang Tang; Shulan Fu

doi:10.3389/fpls.2022.928014

The Physical Location of Stripe Rust Resistance Genes on Chromosome 6 of Rye (Secale cereale L.) AR106BONE

Front Plant Sci. 2022 Jun 29:13:928014. doi: 10.3389/fpls.2022.928014. eCollection 2022.

Authors

Yanling Duan^{1

2}, Jie Luo^{1

2}, Zujun Yang³, Guangrong Li³, Zongxiang Tang^{1

2}, Shulan Fu^{1

2}

Affiliations

¹ College of Agronomy, Sichuan Agricultural University, Chengdu, China.
² Provincial Key Laboratory for Plant Genetics and Breeding, Sichuan Agricultural University, Chengdu, China.
³ Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.

Abstract

It was reported that the chromosome 6R of rye (Secale cereale L.) carries stripe rust resistance gene Yr83, and the region with the candidate resistance gene(s) still needs to be narrowed down. This study confirmed that the chromosome 6RL^Ar derived from rye AR106BONE contains stripe rust resistance gene(s). A wheat-rye T6BS.6RL^Ar translocation chromosome, a wheat-rye small-segment translocation T6RL^Ar-6AS.6AL, and three kinds of deleted T6BS.6RL^Ar translocations, T6BS.6RL^Ar-1, T6BS.6RL^Ar-2, and T6BS.6RL^Ar-3, were identified. Translocations T6BS.6RL^Ar, T6BS.6RL^Ar-2, and T6RL^Ar-6AS.6AL were highly resistant to stripe rust and T6BS.6RL^Ar-1 and T6BS.6RL^Ar-3 were highly susceptible. The molecular markers specific to 6RL determined that the three regions of the 6RL^Ar arm from 732,999,830 bp to the telomere, from 735,010,030 to 848,010,414 bp, and from 848,011,262 bp to the telomere were deleted from T6BS.6RL^Ar-1, T6BS.6RL^Ar-2, and T6BS.6RL^Ar-3, respectively. T6BS.6RL^Ar-2 and T6RL^Ar-6AS.6AL contained the segment that was deleted in T6BS.6RL^Ar-3. Therefore, it can be concluded that about 37 Mb segment from 848,011,262 bp to the telomere carried stripe rust resistance gene(s), and it was smaller than that with the Yr83 gene. Gene annotation indicated that about 37 Mb region contains 43 potential resistance genes, and 42 of them are nucleotide-binding site and leucine-rich repeat (NBS-LRR)-like resistance protein genes. The results in this study narrowed down the size of the region with candidate stripe rust resistance gene(s) on the 6RL arm, and the T6RL^Ar-6AS.6AL is a promising small-segment translocation for improvement of wheat cultivars.

Keywords: 6R chromosome; rye; small-segment translocation; stripe rust; wheat.