Characterization of the complete mitochondrial genome sequence of Coilia brachygnathus (Clupeiformes, Engraulidae) from Huai River and its phylogenetic position

Yuan Tian; Tiezhu Yang; Shijie Yang; Leping Wang; Liangjie Zhao; Wenhao Sun

doi:10.1080/23802359.2022.2097487

Characterization of the complete mitochondrial genome sequence of Coilia brachygnathus (Clupeiformes, Engraulidae) from Huai River and its phylogenetic position

Mitochondrial DNA B Resour. 2022 Jul 12;7(7):1288-1290. doi: 10.1080/23802359.2022.2097487. eCollection 2022.

Authors

Yuan Tian^{1

2}, Tiezhu Yang^{1

2}, Shijie Yang³, Leping Wang⁴, Liangjie Zhao^{1

2}, Wenhao Sun^{1

2}

Affiliations

¹ School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang, China.
² Fishery Biological Engineering Technology Research Center of Henan Province, Xinyang, China.
³ Xinyang Nanwan Reservoir Fishery Development Co. Ltd., Xinyang, China.
⁴ Bureau of Agriculture and Rural Affairs of the Zhumadian City, Zhumadian, China.

Abstract

The complete mitogenome sequence of Coilia brachygnathus (Kreyenberg & Pappenheim, 1908) from Wabu Lake in Huai River Basin was annotated and characterized in this study. This mitochondrial genome is a circular DNA molecule of 16.896 bp in size with 57.52% AT content, including 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and an AT-rich region (control region) as other bony fishes. There are a total of 10 overlap locations and 15 intergenic spacer regions throughout the mitogenome of C. brachygnathus. All PCGs employed a standard ATG as a start codon, except cytochrome c oxidase 1 (cox1) with GTG. In addition, TAA or TAG was identified as the typical stop codon. A phylogenetic tree reconstructed with the maximum likelihood method depicted a clone relationship with eight species of genus Coilia and our previous study based on the amino acid sequences of 13 mitochondrial PCGs. The complete mitochondrial genome is a valuable resource in classifying and conserving C. brachygnathus.

Keywords: Coilia brachygnathus; Huai River; mitochondrial genome; phylogenetic analysis.

Grants and funding

The study was supported by the Youth Fund Project of Xinyang Agriculture and Forestry College [Nos. QN2021020 and 2019LG003], Foundation of Central Laboratory of Xinyang Agriculture and Forestry University [FCL202006], and Doctoral Research Initiation Project [No. 203071].