Investigating the impacts of H2O2 treatment on gills of healthy Atlantic salmon reveals potential changes to mucus production with implications on immune activity

Abstract

Current treatment strategies for relevant infectious diseases in Atlantic salmon (Salmo salar L.) include the use of low salinity or freshwater bathing. However, often availability is restricted, and hydrogen peroxide (H₂O₂) is used as an alternative. The potential impacts of H₂O₂ on fish mucosal tissues, especially the gills therefore need to be considered. In this study the mucosal and immunological effects of H₂O₂ treatment on the gills of healthy Atlantic salmon were examined by gene expression (qPCR) and immunohistochemistry (IHC) investigating T-cell, B-cell, and mucin activity. Healthy fish were treated with H₂O₂ and sampled at different times: 4 h, 24 h and 14 days post-H₂O₂ treatment (dpt) (total n = 18) to investigate the effect of holding time and H₂O₂ treatment. Treatment with H₂O₂ resulted in up-regulation of markers for T-cell activity and anti-inflammatory response and down-regulation of mucin expression in the gills at 14 dpt compared to fish sampled prior to treatment (0h; n = 5 fish). These findings were supported by IHC analysis, which despite being highly variable between samples, showed an increase in the number of CD3⁺ T cells at 14 dpt in 50% of treated fish compared to pre-treatment fish. The results from this study suggest that H₂O₂ treatment does not immune compromise healthy Atlantic salmon after 14 dpt (i.e., post-recovery) but modulates gill immune activity and disrupts the mucus covering of the gills. However, further studies are required to determine whether the effects observed are related to H₂O₂ treatment in isolation or other variables such as holding time or environmental factors.