Gold and silver nanoclusters (Au/AgNCs) exhibit excellent application potential in optical biosensors because of their low toxicity, excellent biocompatibility, and unique optical properties. Au/AgNCs-based visual analysis methods have emerged as powerful tools for detecting various targets with convenient readout. In this review, the applications of Au/AgNCs in the visual detection and bioimaging of metal ions, inorganic anions, small molecules, and biomacromolecules in various devices are summarized. Furthermore, this review also discusses the future perspectives of the field.