Establishment of SIAISi021-A, an induced pluripotent stem cell (iPSC) line from a 71-year-old Chinese Han male with Alzheimer's Disease (AD) having two copies of APOE4/4 allele

Yanfei Ding; Haijuan Chen; Jian Zhao; Ying Wang; Yulei Deng

doi:10.1016/j.scr.2022.102866

Establishment of SIAISi021-A, an induced pluripotent stem cell (iPSC) line from a 71-year-old Chinese Han male with Alzheimer's Disease (AD) having two copies of APOE4/4 allele

Stem Cell Res. 2022 Aug:63:102866. doi: 10.1016/j.scr.2022.102866. Epub 2022 Jul 14.

Authors

Yanfei Ding¹, Haijuan Chen¹, Jian Zhao², Ying Wang², Yulei Deng³

Affiliations

¹ Department of Neurology & Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Neurology, Ruijin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
² Lab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and Technology, ShanghaiTech, China.
³ Department of Neurology & Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Neurology, Ruijin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: wangying1@shanghaitech.edu.cn.

PMID: 35843023
DOI: 10.1016/j.scr.2022.102866

Abstract

A 71-year-old Han male from China contributed peripheral blood mononuclear cells (PBMCs). Non-integrative Sendai viral vectors containing reprogramming factors OCT4, KLF4, SOX2 and C-MYC were used to reprogram PBMCs. Pluripotency makers confirmed the pluripotency of transgene-free induced pluripotent stem cell (iPSC). The ability of iPSC to spontaneously differentiate three germ layers in vitro confirmed the pluripotency of iPSC. The iPSC line displayed a normal karyotype. The newly generated human iPSC SIAISi021-A can be used for studying further disease mechanisms of Alzheimer's Disease (AD).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Alleles
Alzheimer Disease* / genetics
Alzheimer Disease* / metabolism
Apolipoprotein E4 / genetics
Apolipoprotein E4 / metabolism
Cell Culture Techniques
Cell Differentiation
Cells, Cultured
Cellular Reprogramming
Humans
Induced Pluripotent Stem Cells* / metabolism
Leukocytes, Mononuclear
Male

Substances

Apolipoprotein E4