Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient

Johanna Lotila; Tanja Hyvärinen; Heli Skottman; Laura Airas; Susanna Narkilahti; Sanna Hagman

doi:10.1016/j.scr.2022.102865

Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient

Stem Cell Res. 2022 Aug:63:102865. doi: 10.1016/j.scr.2022.102865. Epub 2022 Jul 11.

Authors

Johanna Lotila¹, Tanja Hyvärinen², Heli Skottman³, Laura Airas⁴, Susanna Narkilahti⁵, Sanna Hagman⁶

Affiliations

¹ Neuroimmunology Research Group, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland; NeuroGroup, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland.
² Neuroimmunology Research Group, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland.
³ Eye Group, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland.
⁴ Clinical Neurosciences, University of Turku and Neurocenter, Turku University Hospital, Turku, Finland.
⁵ NeuroGroup, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland.
⁶ Neuroimmunology Research Group, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland. Electronic address: sanna.hagman@tuni.fi.

PMID: 35843021
DOI: 10.1016/j.scr.2022.102865

Abstract

Multiple sclerosis (MS) is a complex autoimmune disease of the central nervous system where the main pathogenetic events include demyelination and axonal degeneration. Here, we generated a human induced pluripotent stem cell (hiPSC) line from peripheral blood mononuclear cells of an MS patient utilizing Sendai virus reprogramming. The produced hiPSC line expressed pluripotency markers, differentiated into three germ layers, showed a normal karyotype and was free of virus vectors, transgenes and mycoplasma. Established hiPSCs are a valuable source for studies of MS disease modeling and drug discovery.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / physiology
Cell Line
Cellular Reprogramming
Humans
Induced Pluripotent Stem Cells* / metabolism
Leukocytes, Mononuclear / metabolism
Multiple Sclerosis* / metabolism