Measurement of ciliary beating and fluid flow in the zebrafish adult telencephalon

Inyoung Jeong; Jan Niklas Hansen; Dagmar Wachten; Nathalie Jurisch-Yaksi

doi:10.1016/j.xpro.2022.101542

Measurement of ciliary beating and fluid flow in the zebrafish adult telencephalon

STAR Protoc. 2022 Sep 16;3(3):101542. doi: 10.1016/j.xpro.2022.101542. Epub 2022 Jul 16.

Authors

Inyoung Jeong¹, Jan Niklas Hansen², Dagmar Wachten², Nathalie Jurisch-Yaksi³

Affiliations

¹ Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Erling Skjalgsons Gate 1, 7491 Trondheim, Norway. Electronic address: inyoung.jung@ntnu.no.
² Institute of Innate Immunity, Biophysical Imaging, Medical Faculty, University of Bonn, 53127 Bonn, Germany.
³ Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Erling Skjalgsons Gate 1, 7491 Trondheim, Norway. Electronic address: nathalie.jurisch-yaksi@ntnu.no.

Abstract

Motile cilia are hair-like structures that move and propel fluid, playing important roles in the physiology of organs. Here, we present a protocol to visualize and measure ciliary beating and cerebrospinal fluid (CSF) flow in the telencephalon of an adult zebrafish brain explant. We describe the preparation of brain explants, the recording of ciliary beating and CSF flow, and data analysis using ImageJ and MATLAB. These imaging and analysis techniques can be directly translated to other ciliated systems. For complete details on the use and execution of this protocol, please refer to D'Gama et al. (2021).

Keywords: Cell Biology; Microscopy; Model Organisms; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / metabolism
Cilia* / metabolism
Telencephalon / metabolism
Zebrafish Proteins / metabolism
Zebrafish* / metabolism

Substances

Zebrafish Proteins