[11]-chaetoglobosins with cytotoxic activities from Pseudeurotium bakeri

Abstract

Fourteen new [11]-chaetoglobosins (1-14), along with two known congeners, cytochalasins X and Y (15 and 16), were isolated from the cultures of an endophytic fungus Pseudeurotium bakeri P1-1-1. Their structures incorporating absolute configurations were elucidated based on the comprehensive analyses of one- and two-dimensional NMR data, HRESIMS spectrometry, chemical methods, and single-crystal X-ray diffraction analysis (Cu Kα). All isolates were evaluated for their cytotoxic activities and chaetopseudeurin M (1) displayed significant cytotoxic effects against seven human cancer cell lines, with IC₅₀ values ranging from 5.1 ± 0.9 to 10.8 ± 0.1 μM. Western blot experiments exhibited that compound 1 exerted its cytotoxic effect in MCF-7 cells by inducing G2/M cell cycle arrest and apoptosis via downregulating the expression of cyclin B1 and Cdk1, and activating Bcl-2/caspase-3/PARP pathway, respectively.

Keywords: Bcl-2/caspase-3/PARP pathway; Cdk1; G2/M cell cycle arrest; Pseudeurotium bakeri P1-1-1; [11]-chaetoglobosins; apoptosis; cyclin B1.