Isolation, Characterization and Anticomplementary Activity of Polysaccharides from the Rhizomes of Belamcanda chinensis (L.) DC

Abstract

The polysaccharides from the rhizomes of Belamcanda chinensis (L.) DC. (BCPs) were obtained by optimal water extraction (extraction temperature 84 °C, liquid to solid ratio 42 mL/g and extraction time 100 min), the extraction yield of BCPs was 23.01±0.27 % (n=3). Furthermore, two novel polysaccharides (BCP-A1 and BCP-B1) were purified by column chromatography. The BCP-A1 (6.0820×10⁴ kDa) was composed of β-D-Manp-(1→, β-D-Glcp-(1→, →4)-α-D-Galp-(1→ and →3,4)-β-D-Galp-(1→, and BCP-B1 (2.2744×10⁴ kDa) was composed of →5)-α-L-Araf-(1→, β-D-Manp-(1→, β-D-Glcp-(1→, →4)-α-D-Glcp, →4)-α-D-Galp-(1→, →4)-α-D-GalpA-(1→ and →3,4)-β-D-Galp-(1→. In anticomplementary experiments, BCP-A1 (CH₅₀ : 0.009±0.003 mg/mL; AP₅₀ : 0.015±0.003 mg/mL) and BCP-B1 (CH₅₀ : 0.004±0.001 mg/mL; AP₅₀ : 0.028±0.005 mg/mL) exhibited potent anticomplementary activity, and acted on C2-, C4- and Factor B components. Our study provides a foundation for BCP-A1 and BCP-B1 as potential complement inhibitors to treat diseases involving with excessive activation of the complement system.

Keywords: Belamcanda chinensis; anticomplementary activity; characterization; polysaccharides.