The significance of thermomechanical processing on the cellular response of biomedical Co-Cr-Mo alloys

Manami Mori; Ting Guo; Kenta Yamanaka; Zuyong Wang; Kazuo Yoshida; Yusuke Onuki; Shigeo Sato; Akihiko Chiba; R D K Misra

doi:10.1016/j.jmbbm.2022.105360

The significance of thermomechanical processing on the cellular response of biomedical Co-Cr-Mo alloys

J Mech Behav Biomed Mater. 2022 Sep:133:105360. doi: 10.1016/j.jmbbm.2022.105360. Epub 2022 Jul 8.

Authors

Manami Mori¹, Ting Guo², Kenta Yamanaka³, Zuyong Wang⁴, Kazuo Yoshida⁵, Yusuke Onuki⁶, Shigeo Sato⁷, Akihiko Chiba⁸, R D K Misra⁹

Affiliations

¹ Department of General Engineering, National Institute of Technology, Sendai College, 48 Nodayama, Medeshima-Shiote, Natori, 981-1239, Japan; Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Japan.
² College of Materials Science and Engineering, College of Biology, Hunan University, Changsha, 410082, P.R. China.
³ Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Japan. Electronic address: kenta.yamanaka.c5@tohoku.ac.jp.
⁴ College of Materials Science and Engineering, College of Biology, Hunan University, Changsha, 410082, P.R. China. Electronic address: wangzy@hnu.edu.cn.
⁵ Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Japan; Eiwa Co., Ltd., 405-45 Kasshi-cho, Kamaishi, 026-0001, Japan.
⁶ Frontier Research Center for Applied Atomic Sciences, Ibaraki University, 162-1 Shirakata, Tokai, 319-1106, Japan.
⁷ Graduate School of Science and Engineering, Ibaraki University, 4-12-1 Nakanarusawa, Hitachi, 316-8511, Japan.
⁸ Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Japan.
⁹ Department of Metallurgical, Materials and Biomedical Engineering, The University of Texas at El Paso, 500 W University Avenue, El Paso, TX, 79968, USA. Electronic address: dmisra2@utep.edu.

PMID: 35839635
DOI: 10.1016/j.jmbbm.2022.105360

Abstract

Strengthening of biomedical Co-Cr-Mo alloys has been explored via thermomechanical processing for enhancing the durability of their biomedical applications. However, the effects of cold and hot deformation on the cellular activity continue to be unclear. In this study, we prepared Co-Cr-Mo alloy rods via cold swaging and hot-caliber rolling and studied the relationship between the microstructure and cellular response of pre-osteoblasts. The cold-swaged rod experienced strain-induced martensitic transformation, which increased the volume fraction of the hexagonal close-packed (hcp) ε-martensite to ∼60 vol.% with an increase in area reduction (r) to 30%. The 111_γ fiber texture of the face-centered cubic (fcc) γ-matrix followed the Shoji-Nishiyama orientation relationship with ε-martensite. Cell culture results revealed beneficial effects of cold swaging on the cell response, in terms of adhesion, proliferation and morphology of cells, although increasing r did not significantly affect cellular metabolism levels. The addition of small content of Zr (0.04 wt.%) led to enhanced focal adhesion of cells, which became more significant at higher r. The microstructural evolution during hot-caliber rolling, namely, grain refinement without any phase transformation and strong texture development, did not appreciably affect the cellular activity. These findings are envisaged to facilitate alloy design and microstructural optimization for favorable tuning the osseointegration of biomedical Co-Cr-Mo alloys.

Keywords: Cellular response; Co–Cr–Mo alloys; Osseointegration; Pre-osteoblasts; Thermomechanical processing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alloys* / chemistry
Biocompatible Materials* / chemistry
Biocompatible Materials* / pharmacology
Materials Testing

Substances

Alloys
Biocompatible Materials