Mass Spectrometry Multiplexed Detection of SARS-CoV-2

Luciana Godoy Viana; Adriana Lebkuchen; Rodrigo Andrade Schuch; Guilherme Gonçalves Okai; Jessica Silva Salgueiro; Karina Helena Morais Cardozo; Valdemir Melechco Carvalho

doi:10.1007/978-1-0716-2395-4_12

Mass Spectrometry Multiplexed Detection of SARS-CoV-2

Methods Mol Biol. 2022:2511:161-174. doi: 10.1007/978-1-0716-2395-4_12.

Authors

Luciana Godoy Viana¹, Adriana Lebkuchen¹, Rodrigo Andrade Schuch¹, Guilherme Gonçalves Okai¹, Jessica Silva Salgueiro², Karina Helena Morais Cardozo¹, Valdemir Melechco Carvalho³

Affiliations

¹ Division of Research and Development, Fleury Group, São Paulo, SP, Brazil.
² Division of Chromatography, Fleury Group, São Paulo, SP, Brazil.
³ Division of Research and Development, Fleury Group, São Paulo, SP, Brazil. valdemir.carvalho@grupofleury.com.br.

PMID: 35838959
DOI: 10.1007/978-1-0716-2395-4_12

Abstract

Testing of large populations for virus infection is now a reality worldwide due to the coronavirus (SARS-CoV-2) pandemic. The demand for SARS-CoV-2 testing using alternatives other than PCR led to the development of mass spectrometry (MS)-based assays. However, MS for SARS-CoV-2 large-scale testing have some downsides, including complex sample preparation and slow data analysis. Here, we describe a high-throughput targeted proteomics method to detect SARS-CoV-2 directly from nasopharyngeal and oropharyngeal swabs. This strategy employs fully automated sample preparation mediated by magnetic particles, followed by detection of SARS-CoV-2 nucleoprotein peptides by turbulent flow chromatography coupled with tandem mass spectrometry.

Keywords: Liquid chromatography tandem mass spectrometry; Multiplexed turbulent flow chromatography; SARS-CoV-2; Targeted proteomics.

MeSH terms

COVID-19 Testing
COVID-19* / diagnosis
Humans
Pandemics
SARS-CoV-2*
Tandem Mass Spectrometry / methods