Live Cell Imaging of ATP Dynamics in Plant Cells

Ryoichi Sato; Shinji Masuda

doi:10.1007/978-1-0716-2473-9_19

Live Cell Imaging of ATP Dynamics in Plant Cells

Methods Mol Biol. 2022:2525:259-266. doi: 10.1007/978-1-0716-2473-9_19.

Authors

Ryoichi Sato¹, Shinji Masuda²

Affiliations

¹ RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan.
² Department of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan. shmasuda@bio.titech.ac.jp.

PMID: 35836074
DOI: 10.1007/978-1-0716-2473-9_19

Abstract

Adenosine triphosphate (ATP) is a central metabolite that functions as the energy currency in a living cell. Therefore, visualizing cellular ATP dynamics provides the fundamental information necessary to understand the molecular events involving life phenomena. Live cell imaging technologies using fluorescence (FL)-based indicators have been developed to analyze the dynamics of various biological processes, such as intracellular ATP synthesis and consumption. However, the application of FL-based indicators to plant cells is limited due to the presence of strong chlorophyll autofluorescence, which drastically worsen the signal-to-noise ratio. The bioluminescent (BL) indicators that do not require excitation light could overcome this problem. In this chapter, we introduce a methodology to analyze ATP dynamics in plant cells using BL ATP indicators.

Keywords: ATP; Arabidopsis; Bioluminescent indicator; Dynamics; Live cell imaging; Plant.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate* / metabolism
Chlorophyll
Fluorescence
Plant Cells* / metabolism

Substances

Chlorophyll
Adenosine Triphosphate