Antibacterial Activity Evaluation of ZnO, CuO, and TiO2 Nanoparticles in Solution and Thin Films

Abstract

This chapter introduces unique methodology of antibacterial activity evaluation of nanoparticles in both solution and thin films. Nanoparticles of ZnO, TiO₂, and CuO are synthesized via the sol-gel method. Antibacterial tests are carried out against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria using disk diffusion and bioluminescence. To perform antibacterial tests on thin films and to overcome bacterial strains recuperation on the supports, a new method of bacterial detaching from the slides is developed based on French standard NF EN 14561.

Keywords: ATP bioluminescence; Antibacterial; CuO; Disk diffusion method; TiO2 nanoparticles; ZnO.