A photocrosslinking antibacterial decellularized matrix hydrogel with nanofiber for cutaneous wound healing

Fan Yu; Atta Ur Rehman Khan; Hui Zheng; Xiaotong Li; Mohamed El-Newehy; Hany El-Hamshary; Yosry Morsi; Jun Li; Jinglei Wu; Xiumei Mo

doi:10.1016/j.colsurfb.2022.112691

A photocrosslinking antibacterial decellularized matrix hydrogel with nanofiber for cutaneous wound healing

Colloids Surf B Biointerfaces. 2022 Sep:217:112691. doi: 10.1016/j.colsurfb.2022.112691. Epub 2022 Jul 8.

Authors

Fan Yu¹, Atta Ur Rehman Khan², Hui Zheng³, Xiaotong Li¹, Mohamed El-Newehy⁴, Hany El-Hamshary⁴, Yosry Morsi⁵, Jun Li⁶, Jinglei Wu⁷, Xiumei Mo⁸

Affiliations

¹ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Songjiang, Shanghai 201600, China.
² Department of Biotechnology, The University of Azad Jammu and Kashmir, Muzaffarabad, Pakistan.
³ Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
⁴ Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
⁵ Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Boroondara, VIC 3122, Australia.
⁶ Department of Orthopedics, Shanghai Tenth People's Hospital, School of medicine, Tongji University, Shanghai 200072, China. Electronic address: lijunspine@163.com.
⁷ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Songjiang, Shanghai 201600, China. Electronic address: jw@dhu.edu.cn.
⁸ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Songjiang, Shanghai 201600, China. Electronic address: xmm@dhu.edu.cn.

PMID: 35834997
DOI: 10.1016/j.colsurfb.2022.112691

Abstract

ddECMMA is the methacrylating product of decellularized dermal extracellular matrix with biological signals and capable of photocrosslinking. Thiolated chitosan (TCS) is an effective antibacterial component. PCLPBA is a kind of plasma-treated polycaprolactone nanofiber dispersions (PCLP) that regulates macrophage polarization and promotes angiogenesis. In this study, we obtained ddECMMA via methacrylation reaction. TCS was prepared by reaction between chitosan and thioglycolic acid. PCLPBA was fabricated via reaction between PCLP and 3-buten-1-amine. TCS and PCLPBA were mixed in ddECMMA solution and photocrosslinked to form DTP4 hydrogel. The hydrogel showed rapid gelation, good mechanical strength, antibacterial and antioxidant properties. When it was cocultured with NIH 3T3 cells, the cells showed good morphology and proliferation rate. After applying it to the full-thickness cutaneous wound, wounds almost healed in 2 weeks via re-epithelialization and neovascularization with negligible scar tissue. The results indicate that DTP4 hydrogel is a promising candidate for clinic skin wound healing.

Keywords: Decellularized matrix; Multifunction; Nanofiber; Photocrosslinking hydrogel; Wound healing.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Chitosan*
Hydrogels / pharmacology
Mice
Nanofibers*
Wound Healing / physiology

Substances

Anti-Bacterial Agents
Hydrogels
Chitosan