Antioxidant Mechanism of Lactiplantibacillus plantarum KM1 Under H2O2 Stress by Proteomics Analysis

Yuan Tian; Yu Wang; Nan Zhang; Minmin Xiao; Jing Zhang; Xinyue Xing; Yue Zhang; Yuling Fan; Xia Li; Bo Nan; Yuhua Wang; Jingsheng Liu

doi:10.3389/fmicb.2022.897387

Antioxidant Mechanism of Lactiplantibacillus plantarum KM1 Under H₂O₂ Stress by Proteomics Analysis

Front Microbiol. 2022 Jun 27:13:897387. doi: 10.3389/fmicb.2022.897387. eCollection 2022.

Authors

Yuan Tian^{1

2}, Yu Wang^{1

2}, Nan Zhang^{1

2}, Minmin Xiao^{1

2}, Jing Zhang^{1

2}, Xinyue Xing^{1

2}, Yue Zhang^{1

2}, Yuling Fan^{1

2}, Xia Li^{1

2}, Bo Nan^{1

2}, Yuhua Wang^{1

2

3

4}, Jingsheng Liu^{1

2

4}

Affiliations

¹ College of Food Science, Jilin Agricultural University, Changchun, China.
² Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun, China.
³ National Processing Laboratory for Soybean Industry and Technology, Changchun, China.
⁴ National Engineering Laboratory for Wheat and Corn Deep Processing, Changchun, China.

Abstract

Lactiplantibacillus plantarum KM1 was screened from natural fermented products, which had probiotic properties and antioxidant function. The survival rate of L. plantarum KM1 was 78.26% at 5 mM H₂O₂. In this study, the antioxidant mechanism of L. plantarum KM1 was deeply analyzed by using the proteomics method. The results demonstrated that a total of 112 differentially expressed proteins (DEPs) were screened, of which, 31 DEPs were upregulated and 81 were downregulated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that DEPs participated in various metabolic pathways such as pyruvate metabolism, carbon metabolism, trichloroacetic acid cycle, amino acid metabolism, and microbial metabolism in diverse environments. These metabolic pathways were related to oxidative stress caused by H₂O₂ in L. plantarum KM1. Therefore, the antioxidant mechanism of L. plantarum KM1 under H₂O₂ stress provided a theoretical basis for its use as a potential natural antioxidant.

Keywords: Lactiplantibacillus plantarum; antioxidant; oxidative stress; probiotic; proteomics.