Near-Infrared Light Regulation of Capture and Release of ctDNA Platforms Based on the DNA Assembly System

Chaihong Gong; Xiaowei Mao; Zhe Wang; Zhang Luo; Zhifan Liu; Yali Ben; Weiying Zhang; Zhenzhong Guo

doi:10.3389/fbioe.2022.891727

Near-Infrared Light Regulation of Capture and Release of ctDNA Platforms Based on the DNA Assembly System

Front Bioeng Biotechnol. 2022 Jun 22:10:891727. doi: 10.3389/fbioe.2022.891727. eCollection 2022.

Authors

Chaihong Gong¹, Xiaowei Mao², Zhe Wang³, Zhang Luo¹, Zhifan Liu¹, Yali Ben³, Weiying Zhang¹, Zhenzhong Guo⁴

Affiliations

¹ School of Life Science, Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Jianghan University, Wuhan, China.
² School of Environment and Health, Jianghan University, Wuhan, China.
³ School of Medicine, Jianghan University, Wuhan, China.
⁴ Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, Wuhan, China.

Abstract

Despite recent progress, a challenge remains on how to gently release and recover viable ctDNA captured on DNA probe-based devices. Here, a reusable detector was successfully manufactured for the capture and release of ctDNA by means of an UCNPs@SiO₂-Azo/CD-probe. Biocompatible NIR light is used to excite UCNPs and convert into local UV light. Continuous irradiation induces a rapid release of the entire ctDNA-probe-CD complex from the functionalized surface via the trans-cis isomerization of azo units without disrupting the ctDNA-structure receptor. Specifically, these composite chips allow reloading DNA probes for reusable ctDNA detection with no obvious influence on their efficiency. The results of our study demonstrated the potential application of this platform for the quantitative detection of ctDNA and the individualized analysis of cancer patients.

Keywords: NIR light-responsive; ctDNA; detection; electrochemical; reversible.