A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution

Nicolas Jacquelot; Qiutong Huang; Gabrielle T Belz; Cyril Seillet

doi:10.1016/j.xpro.2022.101534

A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution

STAR Protoc. 2022 Sep 16;3(3):101534. doi: 10.1016/j.xpro.2022.101534. Epub 2022 Jul 12.

Authors

Nicolas Jacquelot¹, Qiutong Huang², Gabrielle T Belz³, Cyril Seillet⁴

Affiliations

¹ Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2M9, Canada. Electronic address: nicolas.jacquelot@uhnresearch.ca.
² The University of Queensland Diamantina Institute, Faculty of Medicine, The University of Queensland, Woolloongabba, QLD 4102, Australia.
³ The University of Queensland Diamantina Institute, Faculty of Medicine, The University of Queensland, Woolloongabba, QLD 4102, Australia; Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia. Electronic address: g.belz@uq.edu.au.
⁴ Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia. Electronic address: seillet@wehi.edu.au.

Abstract

Innate lymphoid cells (ILCs) and adaptive T cells remain a challenge to study because of a significant overlap in their transcriptomic profiles. Here, we describe the adoptive transfer of ILC progenitors into mice genetically deficient in innate and adaptive immune cells to allow detailed study of the development and function of ILCs and gene regulation in an in vivo setting. For complete details on the use and execution of this protocol, please refer to Jacquelot et al. (2021) and Seillet et al. (2016).

Keywords: Cell Biology; Cell isolation; Flow Cytometry/Mass Cytometry; Immunology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Marrow
Immunity, Innate*
Lymphocytes*
Lymphoid Progenitor Cells
Mice
T-Lymphocytes