Evaluation of SARS-CoV-2 antibody persistence and viral spread in stool: a long-term care experience before COVID-19 vaccination

Oya Ozlem Eren-Kutsoylu; Ozgur Appak; Arzu Nazli-Zeka; Gokcen Omeroglu-Simsek; Nil Tekin; Basak Bayram; Ayca Arzu Sayiner; Nesim Tanglay; Vildan Avkan-Oguz

doi:10.1007/s11845-022-03095-7

Evaluation of SARS-CoV-2 antibody persistence and viral spread in stool: a long-term care experience before COVID-19 vaccination

Ir J Med Sci. 2023 Feb;192(1):263-268. doi: 10.1007/s11845-022-03095-7. Epub 2022 Jul 12.

Authors

Oya Ozlem Eren-Kutsoylu¹, Ozgur Appak², Arzu Nazli-Zeka³, Gokcen Omeroglu-Simsek⁴, Nil Tekin⁵, Basak Bayram⁶, Ayca Arzu Sayiner², Nesim Tanglay⁷, Vildan Avkan-Oguz³

Affiliations

¹ Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey. oyaeren76@yahoo.com.
² Division of Medical Virology, Department of Medical Microbiology, Dokuz Eylul University Faculty of Medicine, Izmir, Turkey.
³ Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey.
⁴ Department of Pulmonary Diseases, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey.
⁵ Narlidere Nursing Home Elderly Care and Rehabilitation Center, Izmir, Turkey.
⁶ Department of Emergency Medicine, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey.
⁷ Turkish Ministry of Family and Social Services, İzmir Provincial Directorate, Izmir, Turkey.

Abstract

Background: Due to elderly residents, nursing homes/assisted living facilities were the most affected places in COVID-19 pandemic. Besides symptomatic patients, asymptomatic patients were detected during routine screening.

Aim: This study aims to determine the factors that affect antibody response and viral shedding in stool samples after natural exposure to the virus in residents and staff who recovered from COVID-19 before the vaccine was available.

Methods: This prospective cross-sectional study was conducted at the nation's highest-capacity Residential and Nursing Home. Blood samples were collected between December 15, 2020 and January 15, 2021 from participating residents and staff for anti-SARS-CoV-2 antibody testing. Stool samples were obtained for SARS-CoV-2 PCR testing 2 months after COVID-19. The Social Sciences (SPSS) program version 15.0 was used for statistical analysis. The Mann-Whitney U test compared SARS-CoV-2 antibody concentration between two groups.

Results: Four hundred sixty-four (52.3%) residents and 424 (47.7%) staff participated. Entirely 259 (29.2%) participants were anti-SARS-CoV-2 IgG (+) and 255 (28.7%) were SARS-CoV-2 PCR (+). Both antibody and PCR positivity was detected in 196 (76.9%). In PCR (-) group, 63 (10.0%) participants were SARS-CoV-2 IgG (+). Antibody titers were found highest in SARS-CoV-2 PCR (+) male residents. SARS-CoV-2 IgG titers were significantly high in SARS-CoV-2 PCR (+) and hospitalized participants regardless of age. Stool samples were obtained from 61(23.9%) participants and were found negative.

Conclusion: A durable SARS-CoV-2 IgG antibody response was monitored at least 9 months after the participants were diagnosed with COVID-19. SARS-CoV-2 antibody positivity was detected 76.9% in PCR (+) and 10.0% in PCR (-) participants. Knowing the duration of detectable antibodies is an important finding for developing disease prevention and public health strategies.

Keywords: Antibody persistence; Natural exposure; Nursing home; Residential care; SARS-CoV-2 antibody; Stool viral shedding.

MeSH terms

Aged
Antibodies, Viral
COVID-19 Vaccines
COVID-19* / diagnosis
COVID-19* / epidemiology
COVID-19* / prevention & control
Cross-Sectional Studies
Humans
Immunoglobulin G
Long-Term Care*
Male
Pandemics
Prospective Studies
SARS-CoV-2
Vaccination

Substances

COVID-19 Vaccines
Antibodies, Viral
Immunoglobulin G

Grants and funding

2470/Dokuz Eylül Üniversitesi