Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging

Nobuo Kitada; Shojiro Maki; Sung-Bae Kim

doi:10.1007/978-1-0716-2453-1_3

Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging

Methods Mol Biol. 2022:2524:37-51. doi: 10.1007/978-1-0716-2453-1_3.

Authors

Nobuo Kitada¹, Shojiro Maki¹, Sung-Bae Kim²

Affiliations

¹ Department of Engineering Science, Graduate School of Informatics and Engineering, The University of Electro-Communications, Chofu, Tokyo, Japan.
² Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan. kimu-sb@aist.go.jp.

PMID: 35821461
DOI: 10.1007/978-1-0716-2453-1_3

Abstract

The present protocol introduces a visible light bioluminescence imaging (BLI) platform together with 12 novel coelenterazine (CTZ) analogues and luciferase sets. We exemplify to create diverse hues of bioluminescence (BL) ranging from blue to far red with the combination of marine luciferases and the three groups of CTZ analogues. We also show how to characterize the new CTZ analogues in detail such as the kinetic parameters, dose dependency, and luciferase specificity. The 2-series CTZ analogues interestingly have specificity to artificial luciferases and are completely dark with Renilla luciferase derivatives in contrast. The 3d is highly specific to only NanoLuc. This BL imaging system covering the visible region provides a useful multicolor imaging portfolio that efficiently images molecular events in mammalian cells.

Keywords: Bioluminescence (BL); Coelenterazine; Imaging; Luciferase; Redshifts.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Light*
Luciferases / genetics
Luciferases, Renilla / genetics
Luminescent Measurements* / methods
Mammals

Substances

Luciferases
nanoluc
Luciferases, Renilla