Lipid metabolism and m6A RNA methylation are altered in lambs supplemented rumen-protected methionine and lysine in a low-protein diet

Kefyalew Gebeyew; Chao Yang; Hui Mi; Yan Cheng; Tianxi Zhang; Fan Hu; Qiongxian Yan; Zhixiong He; Shaoxun Tang; Zhiliang Tan

doi:10.1186/s40104-022-00733-z

Lipid metabolism and m⁶A RNA methylation are altered in lambs supplemented rumen-protected methionine and lysine in a low-protein diet

J Anim Sci Biotechnol. 2022 Jul 13;13(1):85. doi: 10.1186/s40104-022-00733-z.

Authors

Kefyalew Gebeyew^{1

2}, Chao Yang^{1

2}, Hui Mi^{1

2}, Yan Cheng^{1

2}, Tianxi Zhang^{1

2}, Fan Hu^{1

2}, Qiongxian Yan^{1

2}, Zhixiong He^{1

2

3}, Shaoxun Tang^{4

5}, Zhiliang Tan^{1

2

3}

Affiliations

¹ CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, 410125, Hunan, China.
² University of Chinese Academy of Science, Beijing, 100049, China.
³ Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha, 410128, Hunan, China.
⁴ CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, 410125, Hunan, China. sxtang@isa.ac.cn.
⁵ University of Chinese Academy of Science, Beijing, 100049, China. sxtang@isa.ac.cn.

Abstract

Background: Methionine or lysine has been reported to influence DNA methylation and fat metabolism, but their combined effects in N6-methyl-adenosine (m⁶A) RNA methylation remain unclarified. The combined effects of rumen-protected methionine and lysine (RML) in a low-protein (LP) diet on lipid metabolism, m⁶A RNA methylation, and fatty acid (FA) profiles in the liver and muscle of lambs were investigated. Sixty-three male lambs were divided into three treatment groups, three pens per group and seven lambs per pen. The lambs were fed a 14.5% crude protein (CP) diet (adequate protein [NP]), 12.5% CP diet (LP), and a LP diet plus RML (LP + RML) for 60 d.

Results: The results showed that the addition of RML in a LP diet tended to lower the concentrations of plasma leptin (P = 0.07), triglyceride (P = 0.05), and non-esterified FA (P = 0.08). Feeding a LP diet increased the enzyme activity or mRNA expression of lipogenic enzymes and decreased lipolytic enzymes compared with the NP diet. This effect was reversed by supplementation of RML with a LP diet. The inclusion of RML in a LP diet affected the polyunsaturated fatty acids (PUFA), n-3 PUFA, and n-6 PUFA in the liver but not in the muscle, which might be linked with altered expression of FA desaturase-1 (FADS1) and acetyl-CoA carboxylase (ACC). A LP diet supplemented with RML increased (P < 0.05) total m⁶A levels in the liver and muscle and were accompanied by decreased expression of fat mass and obesity-associated protein (FTO) and alkB homologue 5 (ALKBH5). The mRNA expressions of methyltransferase-like 3 (METTL3) and methyltransferase-like 14 (METTL14) in the LP + RML diet group were lower than those in the other two groups. Supplementation of RML with a LP diet affected only liver YTH domain family (YTHDF2) proteins (P < 0.05) and muscle YTHDF3 (P = 0.09), which can be explained by limited m⁶A-binding proteins that were mediated in mRNA fate.

Conclusions: Our findings showed that the inclusion of RML in a LP diet could alter fat deposition through modulations of lipogenesis and lipolysis in the liver and muscle. These changes in fat metabolism may be associated with the modification of m⁶A RNA methylation. A systematic graph illustrates the mechanism of dietary methionine and lysine influence on lipid metabolism and M⁶A. The green arrow with triangular heads indicates as activation and brown-wine arrows with flat heads indicates as suppression.

Keywords: Lambs; Lipid metabolism; Low-protein; Lysine; Methionine; m6A RNA methylation.