Sulfur heterogeneity: A non-negligible factor in manipulating growth and lipid accumulation of Scenedesmus obliquus at a relatively high ratio of carbon to nitrogen

Abstract

Algal biodiesel has been becoming a focus in the field of bioenergy worldwide. In this study, effects of heterogeneous sulfur (SO₄^2-, SO₃^2- and S^2-) on growth and lipid accumulation of Scenedesmus obliquus cultured in wastewater with a C/N ratio of 30 were investigated, respectively. The results shown that SO₄^2-, the optimal sulfur source, could trigger cell growth in a concentration-dependent manner. However, SO₃^2- was superior to the others in boosting carbon uptake of cells, which was subject to NH₄⁺-N concentration. Only SO₄^2- could simultaneously increase lipid content and productivity of cells with a dominant component of oleic acid (C18:1n9c) occupying approximately 40% in fatty acid profile. Additionally, the genes encoding enzymes such as CDIPT, ADPRM, DPP1, pmtA and BTA1 involved in the uppermost lipid-related pathway (glycerophospholipid metabolism) were identified facing different sulfur source regardless of the concentration changes. These findings may facilitate nutrition management efforts to enhance microalgae-based biofuel production.

Keywords: Heterogeneous sulfur; Lipid synthesis; Nutrients uptake; Scenedesmus obliquus; Transcriptome.